The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

R.C. Martin, M. Asahina, P.-P. Liu, J.R. Kristof, J.L. Coppersmith, W.E. Pluskota, G.W. Bassel, N.A. Goloviznina, T.T. Nguyen, C. Martínez-Andújar, M.B. Arun Kumar, P. Pupel, H. Nonogaki

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156SPL13miR172SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.
Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalSeed Science Research
Volume20
Issue number2
DOIs
Publication statusPublished - 1 Jun 2010

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