The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

R.C. Martin; M. Asahina; P.-P. Liu; J.R. Kristof; J.L. Coppersmith; W.E. Pluskota; G.W. Bassel; N.A. Goloviznina; T.T. Nguyen; C. Martínez-Andújar; M.B. Arun Kumar; P. Pupel; H. Nonogaki

doi:10.1017/S0960258510000085

The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

R.C. Martin, M. Asahina, P.-P. Liu, J.R. Kristof, J.L. Coppersmith, W.E. Pluskota, G.W. Bassel, N.A. Goloviznina, T.T. Nguyen, C. Martínez-Andújar, M.B. Arun Kumar, P. Pupel, H. Nonogaki

Biosciences

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Abstract

MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156SPL13miR172SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.

Original language	English
Pages (from-to)	79-87
Number of pages	9
Journal	Seed Science Research
Volume	20
Issue number	2
DOIs	https://doi.org/10.1017/S0960258510000085
Publication status	Published - 1 Jun 2010

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Martin, R. C., Asahina, M., Liu, P-P., Kristof, J. R., Coppersmith, J. L., Pluskota, W. E., Bassel, G. W., Goloviznina, N. A., Nguyen, T. T., Martínez-Andújar, C., Arun Kumar, M. B., Pupel, P., & Nonogaki, H. (2010). The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis. Seed Science Research, 20(2), 79-87. https://doi.org/10.1017/S0960258510000085

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title = "The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis",

abstract = "MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156SPL13miR172SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.",

author = "R.C. Martin and M. Asahina and P.-P. Liu and J.R. Kristof and J.L. Coppersmith and W.E. Pluskota and G.W. Bassel and N.A. Goloviznina and T.T. Nguyen and C. Mart{\'i}nez-And{\'u}jar and {Arun Kumar}, M.B. and P. Pupel and H. Nonogaki",

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Martin, RC, Asahina, M, Liu, P-P, Kristof, JR, Coppersmith, JL, Pluskota, WE, Bassel, GW, Goloviznina, NA, Nguyen, TT, Martínez-Andújar, C, Arun Kumar, MB, Pupel, P & Nonogaki, H 2010, 'The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis', Seed Science Research, vol. 20, no. 2, pp. 79-87. https://doi.org/10.1017/S0960258510000085

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AU - Asahina, M.

AU - Liu, P.-P.

AU - Kristof, J.R.

AU - Coppersmith, J.L.

AU - Pluskota, W.E.

AU - Bassel, G.W.

AU - Goloviznina, N.A.

AU - Nguyen, T.T.

AU - Martínez-Andújar, C.

AU - Arun Kumar, M.B.

AU - Pupel, P.

AU - Nonogaki, H.

PY - 2010/6/1

Y1 - 2010/6/1

N2 - MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156SPL13miR172SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.

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The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

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