The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis

Leigh Breen, A Philp, OC Witard, Sarah Jackman, A Selby, K Smith, K Baar, Kevin Tipton

Research output: Contribution to journalArticle

100 Citations (Scopus)


The aim of the present study was to determine mitochondrial and myofibrillar muscle protein synthesis (MPS) when carbohydrate (CHO) or carbohydrate plus protein (C+P) beverages were ingested following prolonged cycling exercise. The intracellular mechanisms thought to regulate MPS were also investigated. In a single-blind, cross-over study, 10 trained cyclists (age 29 +/- 6 years, ((V) over dot(O3max)) 66.5 +/- 5.1 ml kg(-1) min(-1)) completed two trials in a randomized order. Subjects cycled for 90 min at 77 +/- 1% ((V) over dot(O3max)) before ingesting a CHO (25 g of carbohydrate) or C+P (25 g carbohydrate + 10 g whey protein) beverage immediately and 30 min post-exercise. A primed constant infusion of L-[ring-C-13(6)] phenylalanine began 1.5 h prior to exercise and continued until 4 h post-exercise. Muscle biopsy samples were obtained to determine myofibrillar and mitochondrial MPS and the phosphorylation of intracellular signalling proteins. Arterialized blood samples were obtained throughout the protocol. Plasma amino acid and urea concentrations increased following ingestion of C+P only. Serum insulin concentration increased more for C+P than CHO. Myofibrillar MPS was similar to 35% greater for C+P compared with CHO (0.087 +/- 0.007 and 0.057 +/- 0.006% h(-1), respectively; P = 0.025). Mitochondrial MPS rates were similar for C+P and CHO (0.082 +/- 0.011 and 0.086 +/- 0.018% h(-1), respectively). mTOR(Ser2448) phosphorylation was greater for C+P compared with CHO at 4 h post-exercise (P <0.05). p70S6K(Thr389) phosphorylation increased at 4 h post-exercise for C+P (P <0.05), whilst eEF2(Thr56) phosphorylation increased by similar to 40% at 4 h post-exercise for CHO only (P <0.01). The present study demonstrates that the ingestion of protein in addition to carbohydrate stimulates an increase in myofibrillar, but not mitochondrial, MPS following prolonged cycling. These data indicate that the increase in myofibrillar MPS for C+P could, potentially, be mediated through p70S6K, downstream of mTOR, which in turn may suppress the rise in eEF2 on translation elongation.
Original languageEnglish
Pages (from-to)4011-4025
Number of pages15
JournalThe Journal of Physiology
Issue number16
Publication statusPublished - 1 Aug 2011


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