TY - JOUR
T1 - The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor
AU - Chamsart, Saedthawat
AU - Hewitt, Christopher
AU - Nienow, Alvin
PY - 2005/5/1
Y1 - 2005/5/1
N2 - The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. It is concluded that variations in agitation, aeration rate, or dO(2) concentrations down to about 1% of saturation do not damage the bacterial cells or cause a significant change in physiological response, as measured by flow cytometry, even though the cell size was slightly reduced.
AB - The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. It is concluded that variations in agitation, aeration rate, or dO(2) concentrations down to about 1% of saturation do not damage the bacterial cells or cause a significant change in physiological response, as measured by flow cytometry, even though the cell size was slightly reduced.
KW - Corynebacterium glutamicum
KW - cell size
KW - agitated bioreactor
KW - fluid mechanical stress
KW - continuous cultivation
KW - flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=23444454667&partnerID=8YFLogxK
U2 - 10.1007/s10529-005-4690-5
DO - 10.1007/s10529-005-4690-5
M3 - Article
SN - 1573-6776
VL - 27
SP - 693
EP - 700
JO - Biotechnology Letters
JF - Biotechnology Letters
ER -