Abstract
Cytokine gene expression was investigated in the germinal centre constituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of nongerminal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IFN-alpha, IFN-beta, IFN-gamma, and TNF-alpha. Frequency of cytokine-producing cells and the cytokine production pattern of single cells were determined by single cell PCR. Furthermore, cytokine protein expression was investigated by immunohistology. Using these methods, we found a strong production of IL-1 beta mRNA and protein in a small percentage of FDC. Germinal centre T cells showed production of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha mRNA. The mRNAs of these cytokines could also be detected at the single cell level; as they were produced in a high percentage of germinal centre T cells, whereas immunohistological staining was negative, we conclude that a high percentage of germinal centre T lymphocytes produce IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha in low concentrations in contrast to T cells outside the germinal centre, in which strong cytokine production in few cells was shown earlier.
Original language | English |
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Pages (from-to) | 344-54 |
Number of pages | 11 |
Journal | Cytokine |
Volume | 7 |
Issue number | 4 |
DOIs | |
Publication status | Published - May 1995 |
Keywords
- Dendritic Cells
- Tumor Necrosis Factor-alpha
- Humans
- Interferons
- Interleukins
- Child
- Cell Separation
- B-Lymphocytes
- RNA, Messenger
- Polymerase Chain Reaction
- Base Sequence
- Palatine Tonsil
- Molecular Sequence Data
- Cytokines
- Flow Cytometry
- Gene Expression Regulation
- Immunoenzyme Techniques
- T-Lymphocytes