TY - JOUR
T1 - The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI
AU - Berlanga, Oscar
AU - Tulasne, D
AU - Snell, DC
AU - Jung, S
AU - Moroi, M
AU - Frampton, Jonathan
AU - Watson, SP
PY - 2002/6/1
Y1 - 2002/6/1
N2 - There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.
AB - There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.
KW - convulxin
KW - collagen
KW - collagen-related peptide (CRP)
KW - glycoprotein VI (GPVI)
KW - FcR gamma-chain
UR - http://www.scopus.com/inward/record.url?scp=0036318095&partnerID=8YFLogxK
U2 - 10.1046/j.1432-1033.2002.02969.x
DO - 10.1046/j.1432-1033.2002.02969.x
M3 - Article
C2 - 12071959
VL - 269
SP - 2951
EP - 2960
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 12
ER -