The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers

T J Mitchell; J M Ketley; D W Burdon; D C Candy; J Stephen

doi:10.1099/00222615-23-3-199

The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers

T J Mitchell, J M Ketley, D W Burdon, D C Candy, J Stephen

Microbes, Infections and Microbiomes

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Clostridium difficile crude toxins and purified toxin A had similar effects on stripped rabbit ileal mucosa in Ussing chambers. Both toxin preparations caused secretion of sodium and chloride ions by increasing serosa to mucosa (s----m) fluxes. Transmural potential difference and resistance decreased after toxin treatment. Onset of changes in electrical measurements and ion fluxes coincided with onset of histological changes. The response to theophylline was greatly reduced in toxin-treated tissue compared with control tissue.

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Journal of Medical Microbiology
Volume	23
Issue number	3
DOIs	https://doi.org/10.1099/00222615-23-3-199
Publication status	Published - May 1987

Keywords

Animals
Bacterial Proteins
Bacterial Toxins
Chlorides
Clostridium
Enterotoxins
Ileum
In Vitro Techniques
Intestinal Mucosa
Male
Rabbits
Sodium
Theophylline

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10.1099/00222615-23-3-199

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title = "The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers",

abstract = "Clostridium difficile crude toxins and purified toxin A had similar effects on stripped rabbit ileal mucosa in Ussing chambers. Both toxin preparations caused secretion of sodium and chloride ions by increasing serosa to mucosa (s----m) fluxes. Transmural potential difference and resistance decreased after toxin treatment. Onset of changes in electrical measurements and ion fluxes coincided with onset of histological changes. The response to theophylline was greatly reduced in toxin-treated tissue compared with control tissue.",

keywords = "Animals, Bacterial Proteins, Bacterial Toxins, Chlorides, Clostridium, Enterotoxins, Ileum, In Vitro Techniques, Intestinal Mucosa, Male, Rabbits, Sodium, Theophylline",

author = "Mitchell, {T J} and Ketley, {J M} and Burdon, {D W} and Candy, {D C} and J Stephen",

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T1 - The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers

AU - Mitchell, T J

AU - Ketley, J M

AU - Burdon, D W

AU - Candy, D C

AU - Stephen, J

PY - 1987/5

Y1 - 1987/5

N2 - Clostridium difficile crude toxins and purified toxin A had similar effects on stripped rabbit ileal mucosa in Ussing chambers. Both toxin preparations caused secretion of sodium and chloride ions by increasing serosa to mucosa (s----m) fluxes. Transmural potential difference and resistance decreased after toxin treatment. Onset of changes in electrical measurements and ion fluxes coincided with onset of histological changes. The response to theophylline was greatly reduced in toxin-treated tissue compared with control tissue.

AB - Clostridium difficile crude toxins and purified toxin A had similar effects on stripped rabbit ileal mucosa in Ussing chambers. Both toxin preparations caused secretion of sodium and chloride ions by increasing serosa to mucosa (s----m) fluxes. Transmural potential difference and resistance decreased after toxin treatment. Onset of changes in electrical measurements and ion fluxes coincided with onset of histological changes. The response to theophylline was greatly reduced in toxin-treated tissue compared with control tissue.

KW - Animals

KW - Bacterial Proteins

KW - Bacterial Toxins

KW - Chlorides

KW - Clostridium

KW - Enterotoxins

KW - Ileum

KW - In Vitro Techniques

KW - Intestinal Mucosa

KW - Male

KW - Rabbits

KW - Sodium

KW - Theophylline

U2 - 10.1099/00222615-23-3-199

DO - 10.1099/00222615-23-3-199

M3 - Article

C2 - 2856844

SN - 0022-2615

VL - 23

SP - 199

EP - 204

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 3

ER -

The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers

Abstract

Keywords

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