The collagen receptor glycoprotein VI promotes platelet-mediated aggregation of β-amyloid

Lili Donner, Laura Mara Toska, Irena Kruger, Sandra Groniger, Ruben Barroso, Alice Burleigh, Diego Mezzano, Susanne Pfeiler, Malte Kelm, Norbert Gerdes, Steve Watson, Yi Sun, Margitta Elvers

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
358 Downloads (Pure)


Cerebral amyloid angiopathy (CAA) and β-amyloid (Aβ) deposition in the brain parenchyma are hallmarks of Alzheimer’s disease (AD). We previously reported that platelets contribute to Aβ aggregation in cerebral vessels by secreting the factor clusterin upon binding of Aβ40 to the fibrinogen receptor integrin αIIbβ3. Here, we investigated the contribution of the collagen receptor GPVI (glycoprotein VI) in platelet-induced amyloid aggregation. Using platelets isolated from GPVI–wild type and GPVI-deficient human donors and mice, we found that Aβ40 bound to GPVI, which induced the release of ATP and fibrinogen, resulting in platelet aggregation. Binding of Aβ40 to integrin αIIbβ3, fibrinogen, and GPVI collectively contributed to the formation of amyloid clusters at the platelet surface. Consequently, blockade of αIIbβ3 or genetic loss of GPVI reduced amyloid fibril formation in cultured platelets and decreased the adhesion of Aβ-activated platelets to injured carotid arteries in mice. Application of losartan to inhibit collagen binding to GPVI resulted in decreased Aβ40-stimulated platelet activation, factor secretion, and platelet aggregation. Furthermore, the application of GPVI- or integrin-blocking antibodies reduced the formation of platelet-associated amyloid aggregates. Our findings indicate that Aβ40 promotes platelet-mediated amyloid aggregation by binding to both GPVI and integrin αIIbβ3. Blocking these pathways may therapeutically reduce amyloid plaque formation in cerebral vessels and the brain parenchyma of patients.

Original languageEnglish
Article number9872
JournalScience signaling
Issue number643
Publication statusPublished - 4 Aug 2020

Bibliographical note

Publisher Copyright:
© 2020 American Association for the Advancement of Science. All rights reserved.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'The collagen receptor glycoprotein VI promotes platelet-mediated aggregation of β-amyloid'. Together they form a unique fingerprint.

Cite this