The coexistence of two blaNDM-5 genes on an IncF plasmid as revealed by nanopore sequencing

Yu Feng; Lu Liu; Alan McNally; Zhiyong Zong

doi:10.1128/AAC.00110-18

The coexistence of two ^blaNDM-5 genes on an IncF plasmid as revealed by nanopore sequencing

Yu Feng, Lu Liu, Alan McNally, Zhiyong Zong

Microbiology and Infection

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Abstract

In a carbapenem-resistantEscherichia coliclinical isolate of sequence type 167, two copies of ^blaNDM-5were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B- type. Both ^blaNDM-5genes were located in 11,065-bp regions flanked by two copies of IS26The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of ^blaNDM-5.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Early online date	12 Feb 2018
DOIs	https://doi.org/10.1128/AAC.00110-18
Publication status	E-pub ahead of print - 12 Feb 2018

Keywords

Journal Article

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Cite this

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abstract = "In a carbapenem-resistantEscherichia coliclinical isolate of sequence type 167, two copies of blaNDM-5were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B- type. Both blaNDM-5genes were located in 11,065-bp regions flanked by two copies of IS26The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of blaNDM-5.",

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AU - McNally, Alan

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AB - In a carbapenem-resistantEscherichia coliclinical isolate of sequence type 167, two copies of blaNDM-5were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B- type. Both blaNDM-5genes were located in 11,065-bp regions flanked by two copies of IS26The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of blaNDM-5.

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