Projects per year
Abstract
Vaccination with purified capsular polysaccharide Vi Ag from Salmonella typhi can protect against typhoid fever, although the mechanism for its efficacy is not clearly established. In this study, we have characterized the B cell response to this vaccine in wild-type and T cell-deficient mice. We show that immunization with typhoid Vi polysaccharide vaccine rapidly induces proliferation in B1b peritoneal cells, but not in B1a cells or marginal zone B cells. This induction of B1b proliferation is concomitant with the detection of splenic Vi-specific Ab-secreting cells and protective Ab in Rag1-deficient B1b cell chimeras generated by adoptive transfer-induced specific Ab after Vi immunization. Furthermore, Ab derived from peritoneal B cells is sufficient to confer protection against Salmonella that express Vi Ag. Expression of Vi by Salmonella during infection did not inhibit the development of early Ab responses to non-Vi Ags. Despite this, the protection conferred by immunization of mice with porin proteins from Salmonella, which induce Ab-mediated protection, was reduced postinfection with Vi-expressing Salmonella, although protection was not totally abrogated. This work therefore suggests that, in mice, B1b cells contribute to the protection induced by Vi Ag, and targeting non-Vi Ags as subunit vaccines may offer an attractive strategy to augment current Vi-based vaccine strategies.
Original language | English |
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Pages (from-to) | 5527-5532 |
Number of pages | 6 |
Journal | Journal of Immunology |
Volume | 189 |
Issue number | 12 |
DOIs | |
Publication status | Published - 15 Dec 2012 |
Keywords
- Animals
- Antibodies, Bacterial
- Antigens, Bacterial
- B-Lymphocyte Subsets
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Peritoneal Cavity
- Peritoneum
- Polysaccharides, Bacterial
- Porins
- Salmonella Vaccines
- Salmonella typhi
- Typhoid Fever
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Dive into the research topics of 'The capsular polysaccharide Vi from Salmonella typhi is a B1b antigen'. Together they form a unique fingerprint.Projects
- 2 Finished
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Activities of Salmonella Flagellin: FliCing Immunity On and Off and from Th1 to Th2
Cunningham, A. (Principal Investigator)
Biotechnology & Biological Sciences Research Council
19/12/08 → 18/12/11
Project: Research Councils
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A Bug in the System: How Does Salmonella Induce Novel B Cell Responses?
Cunningham, A. (Principal Investigator)
27/10/08 → 26/10/11
Project: Research Councils