TY - JOUR
T1 - The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination
AU - Kerzendorfer, C
AU - Vignard, J
AU - Pedrosa-Harand, A
AU - Siwiec, T
AU - Akimcheva, S
AU - Jolivet, S
AU - Sablowski, R
AU - Armstrong, Susan
AU - Schweizer, D
AU - Mercier, R
AU - Schlogelhofer, P
PY - 2006/6/15
Y1 - 2006/6/15
N2 - Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.
AB - Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.
UR - http://www.scopus.com/inward/record.url?scp=33746052092&partnerID=8YFLogxK
U2 - 10.1242/jcs.02967
DO - 10.1242/jcs.02967
M3 - Article
C2 - 16763194
SN - 1477-9137
VL - 119
SP - 2486
EP - 2496
JO - Journal of Cell Science
JF - Journal of Cell Science
ER -