Termination of DNA replication forks: "Breaking up is hard to do"

Rachael Bailey, Sara Priego Moreno, Agnieszka Gambus

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighbouring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

Original languageEnglish
Pages (from-to)187-196
JournalNucleus (Austin)
Issue number3
Early online date2 Apr 2015
Publication statusE-pub ahead of print - 2 Apr 2015


  • replicative helicase
  • Xenopus
  • DNA replication
  • Mcm2–7
  • p97 segregase
  • replication termination
  • replisome
  • ubiquitin


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