tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Daniel J Nieves; Geva Hilzenrat; Jason Tran; Zhengmin Yang; Hugh H MacRae; Matthew A B Baker; J Justin Gooding; Katharina Gaus

doi:10.1101/604462

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Daniel J Nieves
, Geva Hilzenrat
, Jason Tran
, Zhengmin Yang
, Hugh H MacRae
, Matthew A B Baker
, J Justin Gooding
, Katharina Gaus

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

222 Downloads (Pure)

Abstract

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

Original language	English
Article number	191268
Journal	Royal Society Open Science
Volume	6
Issue number	12
DOIs	https://doi.org/10.1101/604462 https://doi.org/10.1098/rsos.191268
Publication status	Published - 11 Dec 2019

Keywords

protein tags
monovalent
covalent
DNA-PAINT imaging
superresolution

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10.1101/604462Licence: Creative Commons: Attribution (CC BY)
10.1098/rsos.191268Licence: Creative Commons: Attribution (CC BY)

Nieves_et_al_2019_tagPAINT_covalent_labelling_Royal_Society_Open_ScienceFinal published version, 732 KBLicence: Creative Commons: Attribution (CC BY)

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
Nieves, D. J., Hilzenrat, G., Tran, J., Yang, Z., MacRae, H. H., Baker, M. A. B., Gooding, J. J. & Gaus, K., 11 Dec 2019, In: Royal Society Open Science. 6, 12, 191268.
Research output: Contribution to journal › Article › peer-review

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3 Citations (Scopus)

222 Downloads (Pure)

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title = "tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging",

abstract = "Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.",

keywords = "protein tags, monovalent, covalent, DNA-PAINT imaging, superresolution",

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AU - Nieves, Daniel J

AU - Hilzenrat, Geva

AU - Tran, Jason

AU - Yang, Zhengmin

AU - MacRae, Hugh H

AU - Baker, Matthew A B

AU - Gooding, J Justin

AU - Gaus, Katharina

PY - 2019/12/11

Y1 - 2019/12/11

N2 - Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

AB - Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

KW - protein tags

KW - monovalent

KW - covalent

KW - DNA-PAINT imaging

KW - superresolution

U2 - 10.1101/604462

DO - 10.1101/604462

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JO - Royal Society Open Science

JF - Royal Society Open Science

IS - 12

M1 - 191268

ER -

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

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Keywords

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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Cite this