Projects per year
Abstract
The interaction of C-type lectin receptor 2 (CLEC-2) on platelets with Podoplanin on lymphatic endothelial cells initiates platelet signaling events that are necessary for prevention of blood-lymph mixing during development. In the present study, we show that CLEC-2 signaling via Src family and Syk tyrosine kinases promotes platelet adhesion to primary mouse lymphatic endothelial cells at low shear. Using supported lipid bilayers containing mobile Podoplanin, we further show that activation of Src and Syk in platelets promotes clustering of CLEC-2 and Podoplanin. Clusters of CLEC-2-bound Podoplanin migrate rapidly to the center of the platelet to form a single structure. Fluorescence lifetime imaging demonstrates that molecules within these clusters are within 10 nm of one another and that the clusters are disrupted by inhibition of Src and Syk family kinases. CLEC-2 clusters are also seen in platelets adhered to immobilized Podoplanin using direct stochastic optical reconstruction microscopy. These findings provide mechanistic insight by which CLEC-2 signaling promotes adhesion to Podoplanin and regulation of Podoplanin signaling, thereby contributing to lymphatic vasculature development.
Original language | English |
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Pages (from-to) | 35695-35710 |
Number of pages | 16 |
Journal | Journal of Biological Chemistry |
Volume | 289 |
Issue number | 52 |
Early online date | 3 Nov 2014 |
DOIs | |
Publication status | Published - 26 Dec 2014 |
Keywords
- Endothelial Cell
- Lipid Bilayer
- Platelet
- Receptor
- Tyrosine-Protein Kinase (Tyrosine Kinase)
- CLEC-2
- ITAM
- Podoplanin
- Src Family Kinase
- Syk
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Dive into the research topics of 'Syk and Src family kinases regulate C-type lectin receptor 2 (clec-2)-mediated clustering of podoplanin and platelet adhesion to lymphatic endothelial cells'. Together they form a unique fingerprint.Projects
- 1 Finished
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The Early Events in Signalling by Platelet ITAM and ITAM-Like Receptors
1/08/09 → 31/03/16
Project: Research