Supramolecular Photoactivatable Anticancer Hydrogels

V. Venkatesh; Narendra Kumar Mishra; Isolda Romero-canelón; Robbin R. Vernooij; Huayun Shi; James P. C. Coverdale; Abraha Habtemariam; Sandeep Verma; Peter J. Sadler

doi:10.1021/jacs.7b00186

Supramolecular Photoactivatable Anticancer Hydrogels

V. Venkatesh, Narendra Kumar Mishra, Isolda Romero-canelón, Robbin R. Vernooij, Huayun Shi, James P. C. Coverdale, Abraha Habtemariam, Sandeep Verma, Peter J. Sadler

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Abstract

A photoactivatable dopamine-conjugated platinum(IV) anticancer complex (Pt-DA) has been incorporated into G-quadruplex G4K+ borate hydrogels by using borate ester linkages (Pt-G4K+B hydrogel). These were characterized by 11B NMR, attenuated total reflection Fourier transform infrared spectroscopy, circular dichroism, scanning electron microscopy and transmission electron microscopy. Microscopy investigations revealed the transformation of an extended fiber assembly into discrete flakes after incorporation of Pt-DA. Pt-DA showed photocytotoxicity against cisplatin-resistant A2780Cis human ovarian cancer cells (IC50 74 μM, blue light) with a photocytotoxic index <2, whereas Pt-G4K+B hydrogels exhibited more potent photocytotoxicity (IC50 3 μM, blue light) with a photocytotoxic index >5. Most notably, Pt-DA and Pt-G4K+B hydrogels show selective phototoxicity for cancer cells versus normal fibroblast cells (MRC5).

Original language	English
Pages (from-to)	5656-5659
Journal	Journal of the American Chemical Society
Volume	139
Issue number	16
Early online date	17 Apr 2017
DOIs	https://doi.org/10.1021/jacs.7b00186
Publication status	Published - 26 Apr 2017

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Venkatesh_et_al_Supramolecular_photoactivatable_Journal_of_the_American_Chemical_Society_2017
Published in Journal of the American Chemical Society on 17/04/2017 DOI: 10.1021/jacs.7b00186
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https://pubs.acs.org/doi/10.1021/jacs.7b00186Licence: Creative Commons: Attribution (CC BY)

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title = "Supramolecular Photoactivatable Anticancer Hydrogels",

abstract = "A photoactivatable dopamine-conjugated platinum(IV) anticancer complex (Pt-DA) has been incorporated into G-quadruplex G4K+ borate hydrogels by using borate ester linkages (Pt-G4K+B hydrogel). These were characterized by 11B NMR, attenuated total reflection Fourier transform infrared spectroscopy, circular dichroism, scanning electron microscopy and transmission electron microscopy. Microscopy investigations revealed the transformation of an extended fiber assembly into discrete flakes after incorporation of Pt-DA. Pt-DA showed photocytotoxicity against cisplatin-resistant A2780Cis human ovarian cancer cells (IC50 74 μM, blue light) with a photocytotoxic index <2, whereas Pt-G4K+B hydrogels exhibited more potent photocytotoxicity (IC50 3 μM, blue light) with a photocytotoxic index >5. Most notably, Pt-DA and Pt-G4K+B hydrogels show selective phototoxicity for cancer cells versus normal fibroblast cells (MRC5).",

author = "V. Venkatesh and Mishra, {Narendra Kumar} and Isolda Romero-canel{\'o}n and Vernooij, {Robbin R.} and Huayun Shi and Coverdale, {James P. C.} and Abraha Habtemariam and Sandeep Verma and Sadler, {Peter J.}",

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AU - Venkatesh, V.

AU - Mishra, Narendra Kumar

AU - Romero-canelón, Isolda

AU - Vernooij, Robbin R.

AU - Shi, Huayun

AU - Coverdale, James P. C.

AU - Habtemariam, Abraha

AU - Verma, Sandeep

AU - Sadler, Peter J.

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AB - A photoactivatable dopamine-conjugated platinum(IV) anticancer complex (Pt-DA) has been incorporated into G-quadruplex G4K+ borate hydrogels by using borate ester linkages (Pt-G4K+B hydrogel). These were characterized by 11B NMR, attenuated total reflection Fourier transform infrared spectroscopy, circular dichroism, scanning electron microscopy and transmission electron microscopy. Microscopy investigations revealed the transformation of an extended fiber assembly into discrete flakes after incorporation of Pt-DA. Pt-DA showed photocytotoxicity against cisplatin-resistant A2780Cis human ovarian cancer cells (IC50 74 μM, blue light) with a photocytotoxic index <2, whereas Pt-G4K+B hydrogels exhibited more potent photocytotoxicity (IC50 3 μM, blue light) with a photocytotoxic index >5. Most notably, Pt-DA and Pt-G4K+B hydrogels show selective phototoxicity for cancer cells versus normal fibroblast cells (MRC5).

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Supramolecular Photoactivatable Anticancer Hydrogels

Abstract

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