Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters

Andrew Sanderson; Jennie Mitchell; Stephen Minchin; Stephen Busby

doi:10.1016/S0014-5793(03)00500-3

Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters

Andrew Sanderson, Jennie Mitchell, Stephen Minchin, Stephen Busby

Biosciences

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36 Citations (Scopus)

Abstract

Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	199-205
Number of pages	7
Journal	FEBS Letters
Volume	544
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00500-3
Publication status	Published - 5 Jun 2003

Keywords

bacterial transcription initiation
sigma(70) subunit
promoter recognition
RNA polymerase
extended-10 promoters

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10.1016/S0014-5793(03)00500-3

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abstract = "Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",

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AU - Sanderson, Andrew

AU - Mitchell, Jennie

AU - Minchin, Stephen

AU - Busby, Stephen

PY - 2003/6/5

Y1 - 2003/6/5

N2 - Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

AB - Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

KW - bacterial transcription initiation

KW - sigma(70) subunit

KW - promoter recognition

KW - RNA polymerase

KW - extended-10 promoters

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DO - 10.1016/S0014-5793(03)00500-3

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VL - 544

SP - 199

EP - 205

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters

Abstract

Keywords

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