Substituted Maleimides: Self-Reportable Linkers and Tags in Bioconjugation, Material Science, and Nanotechnology

Substituted maleimides are customisable fluorescent linkers and probes with adaptable reactivity and optical properties. Their compact nature and tunability has led to their use in various applications. For example, their solvatochromic properties offer real-time feedback on linking chemistry and environmental changes, essential for applications in material labelling, drug delivery, and nanoparticle functionalization. This review focuses on developing, synthesising, and modifying substituted maleimides as environment-sensitive fluorescent linkers and probes. It delves into their photophysical dynamics and strategic applications, highlighting their significant contributions to macromolecule conjugation and the development of self-reporting materials.