Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System

Mokhaled Mohammed; Peter Thurgood; Christopher Gilliam; Ngan Nguyen; Elena Pirogova; Karlheinz Peter; Khashayar Khoshmanesh; Sara Baratchi

doi:10.1021/acs.analchem.9b03247

Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System

Mokhaled Mohammed, Peter Thurgood, Christopher Gilliam, Ngan Nguyen, Elena Pirogova, Karlheinz Peter, Khashayar Khoshmanesh, Sara Baratchi^*

^*Corresponding author for this work

Electronic, Electrical and Systems Engineering

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We describe a piezoelectric pumping system for studying the mechanobiology of human aortic endothelial cells (HAECs) under pulsatile flow in microfluidic structures. The system takes advantage of commercially available components, including pumps, flow sensors, and microfluidic channels, which can be easily integrated, programmed, and operated by cellular biologists. Proof-of-concept experiments were performed to elucidate the complex mechanotransduction processes of endothelial cells to pulsatile flow. In particular, we investigated the effect of atheroprone and atheroprotective pulsatile shear stress on endothelial cytoskeleton remodeling and distribution of β-catenin, as well as nuclear shape and size. The system is simple to operate, relatively inexpensive, portable, and controllable, providing opportunities for studying the mechanobiology of endothelial cells using microfluidic technologies.

Original language	English
Pages (from-to)	12077-12084
Number of pages	8
Journal	Analytical Chemistry
Volume	91
Issue number	18
DOIs	https://doi.org/10.1021/acs.analchem.9b03247
Publication status	Published - 17 Sept 2019

Bibliographical note

Funding Information:
E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding “The Australian Centre for Electromagnetic Bioeffects Research”’ (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239).

Funding Information:
E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding "The Australian Centre for Electromagnetic Bioeffects Research"' (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239).

Publisher Copyright:
Copyright © 2019 American Chemical Society.

ASJC Scopus subject areas

Analytical Chemistry

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title = "Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System",

abstract = "We describe a piezoelectric pumping system for studying the mechanobiology of human aortic endothelial cells (HAECs) under pulsatile flow in microfluidic structures. The system takes advantage of commercially available components, including pumps, flow sensors, and microfluidic channels, which can be easily integrated, programmed, and operated by cellular biologists. Proof-of-concept experiments were performed to elucidate the complex mechanotransduction processes of endothelial cells to pulsatile flow. In particular, we investigated the effect of atheroprone and atheroprotective pulsatile shear stress on endothelial cytoskeleton remodeling and distribution of β-catenin, as well as nuclear shape and size. The system is simple to operate, relatively inexpensive, portable, and controllable, providing opportunities for studying the mechanobiology of endothelial cells using microfluidic technologies.",

author = "Mokhaled Mohammed and Peter Thurgood and Christopher Gilliam and Ngan Nguyen and Elena Pirogova and Karlheinz Peter and Khashayar Khoshmanesh and Sara Baratchi",

note = "Funding Information: E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding “The Australian Centre for Electromagnetic Bioeffects Research”{\textquoteright} (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239). Funding Information: E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding {"}The Australian Centre for Electromagnetic Bioeffects Research{"}' (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239). Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",

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Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System

Abstract

Bibliographical note

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