Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System

Mokhaled Mohammed, Peter Thurgood, Christopher Gilliam, Ngan Nguyen, Elena Pirogova, Karlheinz Peter, Khashayar Khoshmanesh, Sara Baratchi

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We describe a piezoelectric pumping system for studying the mechanobiology of human aortic endothelial cells (HAECs) under pulsatile flow in microfluidic structures. The system takes advantage of commercially available components, including pumps, flow sensors, and microfluidic channels, which can be easily integrated, programmed, and operated by cellular biologists. Proof-of-concept experiments were performed to elucidate the complex mechanotransduction processes of endothelial cells to pulsatile flow. In particular, we investigated the effect of atheroprone and atheroprotective pulsatile shear stress on endothelial cytoskeleton remodeling and distribution of β-catenin, as well as nuclear shape and size. The system is simple to operate, relatively inexpensive, portable, and controllable, providing opportunities for studying the mechanobiology of endothelial cells using microfluidic technologies.

Original languageEnglish
Pages (from-to)12077-12084
Number of pages8
JournalAnalytical Chemistry
Volume91
Issue number18
DOIs
Publication statusPublished - 17 Sep 2019

Bibliographical note

Funding Information:
E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding “The Australian Centre for Electromagnetic Bioeffects Research”’ (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239).

Funding Information:
E.P. acknowledges the Australian National Health and Medical Research Council (NHMRC) for funding "The Australian Centre for Electromagnetic Bioeffects Research"' (NHMRC CRE APP1135076). K.P. acknowledges NHMRC for a Principal Research Fellowship (GNT1079492). K.K. acknowledges the Australian Research Council (ARC) for a Discovery Grant (DP180102049). S.B. acknowledges ARC for a Discovery for Early Career Researchers Award (DE170100239).

Publisher Copyright:
Copyright © 2019 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint

Dive into the research topics of 'Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System'. Together they form a unique fingerprint.

Cite this