Structural investigation of hemicelluloses from Plantago ovata, Mimosa pudica and Lallemantia royleana by MALDI-ToF mass spectrometry

Fozia Iram; Shazma Massey; Mohammad S. Iqbal; Douglas G. Ward

doi:10.1080/07328303.2018.1487973

Structural investigation of hemicelluloses from Plantago ovata, Mimosa pudica and Lallemantia royleana by MALDI-ToF mass spectrometry

Fozia Iram, Shazma Massey, Mohammad S. Iqbal^*, Douglas G. Ward

^*Corresponding author for this work

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

This work concerns structural investigation of water-extractable hemicelluloses from seed husk of Plantago ovata and seeds of Mimosa pudica and Lallemantia royleana. The composition of the materials was determined by CHN elemental analysis, FT-IR spectrometry and monosaccharide analysis. The detailed structural analysis was carried out by MALDI-ToF mass spectrometry. The absence of nitrogen in the materials suggested that they were free from proteins. The isolated materials were found to be branched hemicelluloses. The mass spectrometric study showed presence of β-1,4-linked xylose with arabinose attached to main chain at β-1,3 positions (Plantago ovata), β-1,4-linked xylose with glucose attached to main chain at β-1,3 positions (Mimosa pudica), and β-1,2-linked rhamnose and β-1,3-linked arabinose units in the main chain with arabinose attached to the main chain through β-1,3-linkage (Lallemantia royleana).

Original language	English
Pages (from-to)	285-301
Number of pages	17
Journal	Journal of Carbohydrate Chemistry
Volume	37
Issue number	5
DOIs	https://doi.org/10.1080/07328303.2018.1487973
Publication status	Published - 13 Jun 2018

Bibliographical note

Funding Information:
FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220].

Funding Information:
FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220]. The authors thank Institute of Cancer and Genomic Sciences, University of Birmingham, UK, for MALDI-ToF analysis and Dr. Bettina Wolf, Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough and Dr. David Coles, School of Biosciences, University of Nottingham, UK, for assistance in monosaccharide analysis.

Publisher Copyright:
© 2018, © 2018 Taylor & Francis Group, LLC.

Keywords

Carbohydrate
hemicellulose
MALDI-ToF
mass spectrometry
polysaccharide
structural analysis

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1080/07328303.2018.1487973

Cite this

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title = "Structural investigation of hemicelluloses from Plantago ovata, Mimosa pudica and Lallemantia royleana by MALDI-ToF mass spectrometry",

abstract = "This work concerns structural investigation of water-extractable hemicelluloses from seed husk of Plantago ovata and seeds of Mimosa pudica and Lallemantia royleana. The composition of the materials was determined by CHN elemental analysis, FT-IR spectrometry and monosaccharide analysis. The detailed structural analysis was carried out by MALDI-ToF mass spectrometry. The absence of nitrogen in the materials suggested that they were free from proteins. The isolated materials were found to be branched hemicelluloses. The mass spectrometric study showed presence of β-1,4-linked xylose with arabinose attached to main chain at β-1,3 positions (Plantago ovata), β-1,4-linked xylose with glucose attached to main chain at β-1,3 positions (Mimosa pudica), and β-1,2-linked rhamnose and β-1,3-linked arabinose units in the main chain with arabinose attached to the main chain through β-1,3-linkage (Lallemantia royleana).",

keywords = "Carbohydrate, hemicellulose, MALDI-ToF, mass spectrometry, polysaccharide, structural analysis",

author = "Fozia Iram and Shazma Massey and Iqbal, {Mohammad S.} and Ward, {Douglas G.}",

note = "Funding Information: FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220]. Funding Information: FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220]. The authors thank Institute of Cancer and Genomic Sciences, University of Birmingham, UK, for MALDI-ToF analysis and Dr. Bettina Wolf, Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough and Dr. David Coles, School of Biosciences, University of Nottingham, UK, for assistance in monosaccharide analysis. Publisher Copyright: {\textcopyright} 2018, {\textcopyright} 2018 Taylor & Francis Group, LLC.",

year = "2018",

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language = "English",

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AU - Massey, Shazma

AU - Iqbal, Mohammad S.

AU - Ward, Douglas G.

N1 - Funding Information: FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220]. Funding Information: FI acknowledges HEC Pakistan under IRSIP for a grant for working at University of Birmingham, UK. SM and MSI acknowledge a research grant from HEC Pakistan [No. 20-3775/NRPU/R&D/HEC/14/1220]. The authors thank Institute of Cancer and Genomic Sciences, University of Birmingham, UK, for MALDI-ToF analysis and Dr. Bettina Wolf, Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough and Dr. David Coles, School of Biosciences, University of Nottingham, UK, for assistance in monosaccharide analysis. Publisher Copyright: © 2018, © 2018 Taylor & Francis Group, LLC.

PY - 2018/6/13

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N2 - This work concerns structural investigation of water-extractable hemicelluloses from seed husk of Plantago ovata and seeds of Mimosa pudica and Lallemantia royleana. The composition of the materials was determined by CHN elemental analysis, FT-IR spectrometry and monosaccharide analysis. The detailed structural analysis was carried out by MALDI-ToF mass spectrometry. The absence of nitrogen in the materials suggested that they were free from proteins. The isolated materials were found to be branched hemicelluloses. The mass spectrometric study showed presence of β-1,4-linked xylose with arabinose attached to main chain at β-1,3 positions (Plantago ovata), β-1,4-linked xylose with glucose attached to main chain at β-1,3 positions (Mimosa pudica), and β-1,2-linked rhamnose and β-1,3-linked arabinose units in the main chain with arabinose attached to the main chain through β-1,3-linkage (Lallemantia royleana).

AB - This work concerns structural investigation of water-extractable hemicelluloses from seed husk of Plantago ovata and seeds of Mimosa pudica and Lallemantia royleana. The composition of the materials was determined by CHN elemental analysis, FT-IR spectrometry and monosaccharide analysis. The detailed structural analysis was carried out by MALDI-ToF mass spectrometry. The absence of nitrogen in the materials suggested that they were free from proteins. The isolated materials were found to be branched hemicelluloses. The mass spectrometric study showed presence of β-1,4-linked xylose with arabinose attached to main chain at β-1,3 positions (Plantago ovata), β-1,4-linked xylose with glucose attached to main chain at β-1,3 positions (Mimosa pudica), and β-1,2-linked rhamnose and β-1,3-linked arabinose units in the main chain with arabinose attached to the main chain through β-1,3-linkage (Lallemantia royleana).

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KW - MALDI-ToF

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ER -

Structural investigation of hemicelluloses from Plantago ovata, Mimosa pudica and Lallemantia royleana by MALDI-ToF mass spectrometry

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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