Stoichiometric quantification of spatially dense assemblies with qPAINT

Matthew A. B. Baker; Daniel J. Nieves; Geva Hilzenrat; Jonathan F. Berengut; Katharina Gaus; Lawrence K. Lee

doi:10.1039/C9NR00472F

Stoichiometric quantification of spatially dense assemblies with qPAINT

Matthew A. B. Baker, Daniel J. Nieves, Geva Hilzenrat, Jonathan F. Berengut, Katharina Gaus, Lawrence K. Lee

Immunology and Immunotherapy

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Abstract

Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

Original language	English
Article number	12460-12464
Pages (from-to)	12460-12464
Number of pages	5
Journal	Nanoscale
Volume	11
Issue number	26
DOIs	https://doi.org/10.1039/C9NR00472F
Publication status	Published - 14 Jul 2019

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10.1039/C9NR00472FLicence: None: All rights reserved

Baker_et_al_Stoichiometric_quantification_of_spatially_dense_assemblies_with_qPAINT_Nanoscale_2019
Baker, M. et al (2019) Stoichiometric quantification of spatially dense assemblies with qPAINT, Nanoscale, volume 11, pages 12460-12464, DOI: 10.1039/C9NR00472F
Accepted author manuscript, 2.78 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivs (CC BY-NC-ND)

https://pubs.rsc.org/en/content/articlelanding/2019/NR/C9NR00472FLicence: None: All rights reserved

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AU - Lee, Lawrence K.

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AB - Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

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Stoichiometric quantification of spatially dense assemblies with qPAINT

Abstract

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