Abstract
In this study the effects of rhIGF-I on macrophage differentiation and growth have been studied using liquid suspension cultures of rat bone marrow cells. IGF-I stimulated macrophage growth in a dose-dependent manner, a maximum response was found at a concentration of 20 ng/ml. IGF-I effects could be ascribed to stimulation of both postmitotic and proliferating cells. A remarkable finding was that IGF-I induced formation of multinucleated cells (MNC). The MNC resembled macrophage-like cells (AcP, NSE positive). A monoclonal antibody to rhIGF-I significantly inhibited IGF-stimulated macrophage growth and MNC formation. A specific antibody to mouse CSF-1 reduced IGF-stimulated macrophage growth in mouse bone marrow cultures indicating that IGF-I effects could, at least in part, be ascribed to endogenous production of CSF-1. These findings indicate that IGF-I in concert with locally induced CSF-1 can influence the differentiation and growth of bone marrow-derived macrophages.
Original language | English |
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Pages (from-to) | 647-53 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 174 |
Issue number | 2 |
Publication status | Published - 31 Jan 1991 |
Keywords
- Macrophages
- Animals
- Recombinant Proteins
- Hematopoietic Stem Cells
- Cell Nucleus
- Insulin-Like Growth Factor I
- Macrophage Colony-Stimulating Factor
- Rats
- Antibodies, Monoclonal
- Bone Marrow Cells
- Antibodies
- Cells, Cultured
- Kinetics
- DNA Replication
- Cell Division