Stimulation of human spermatozoa with progesterone gradients to simulate approach to the oocyte. Induction of (Ca(2+))(1) oscillations and cyclical transitions in flagellar beating

CV Harper, Christopher Barratt, Stephen Publicover

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

Progesterone is present at micromolar concentrations in the cumulus matrix, which surrounds mammalian oocytes. Exposure of human spermatozoa to a concentration gradient of progesterone (0-3 microM) to simulate approach to the oocyte induced a slowly developing increase in [Ca(2+)](i) upon which, in many cells, slow oscillations were superimposed. [Ca(2+)](i) oscillations often started at very low progesterone (
Original languageEnglish
Pages (from-to)46315-46325
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number44
DOIs
Publication statusPublished - 11 Aug 2004

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