TY - JOUR
T1 - Stepwise enzymatic dephosphorylation of inositol 1,4,5-trisphosphate to inositol in liver
AU - Storey, Diane J.
AU - Shears, Stephen B.
AU - Kirk, Christopher J.
AU - Michell, Robert H.
PY - 1984/12/1
Y1 - 1984/12/1
N2 - Many receptors for hormones, neurotransmitters and other signals cause hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and effect a rise in cytosolic Ca2+ concentration1-7. The inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) liberated during PtdIns(4,5)P2 breakdown seems to serve as a second messenger that activates the release of Ca2+ from a nonmitochondrial intracellular compartment7-12. As expected if it is an important intracellular messenger, Ins(1,4,5)P3 is relatively rapidly degraded, both within stimulated cells13,14 and when added to homogenates of blowfly salivary gland15 or to permeabilized, but not intact, hepatocytes10. Here we report that the dephosphorylation reactions responsible for the conversion of Ins(1,4,5)P3 to free inositol in rat liver are catalysed by two or more enzymes, and that these reactions are distributed between the plasma membrane and cytosol. The Ins(1,4,5)P3 5-phosphatase and inositol 1-phosphate (Ins(1)P) phosphatase of liver appear similar to enzymes described previously in erythrocytes16 and brain17.
AB - Many receptors for hormones, neurotransmitters and other signals cause hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and effect a rise in cytosolic Ca2+ concentration1-7. The inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) liberated during PtdIns(4,5)P2 breakdown seems to serve as a second messenger that activates the release of Ca2+ from a nonmitochondrial intracellular compartment7-12. As expected if it is an important intracellular messenger, Ins(1,4,5)P3 is relatively rapidly degraded, both within stimulated cells13,14 and when added to homogenates of blowfly salivary gland15 or to permeabilized, but not intact, hepatocytes10. Here we report that the dephosphorylation reactions responsible for the conversion of Ins(1,4,5)P3 to free inositol in rat liver are catalysed by two or more enzymes, and that these reactions are distributed between the plasma membrane and cytosol. The Ins(1,4,5)P3 5-phosphatase and inositol 1-phosphate (Ins(1)P) phosphatase of liver appear similar to enzymes described previously in erythrocytes16 and brain17.
UR - http://www.scopus.com/inward/record.url?scp=0021680122&partnerID=8YFLogxK
U2 - 10.1038/312374a0
DO - 10.1038/312374a0
M3 - Article
C2 - 6095097
AN - SCOPUS:0021680122
SN - 0028-0836
VL - 312
SP - 374
EP - 376
JO - Nature
JF - Nature
IS - 5992
ER -