Starch-chitosan polyplexes: a versatile carrier system for anti-infectives and gene delivery

Hanzey Yasar, Duy Khiet Ho, Chiara De Rossi, Jennifer Herrmann, Sarah Gordon, Brigitta Loretz, Claus Michael Lehr

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)
1 Downloads (Pure)

Abstract

Despite the enormous potential of nanomedicine, the search for materials from renewable resources that balance bio-medical requirements and engineering aspects is still challenging. This study proposes an easy method to make nanoparticles composed of oxidized starch and chitosan, both isolated from natural biopolymers. The careful adjustment of C/N ratio, polymer concentration and molecular weight allowed for tuning of particle characteristics. The system's carrier capability was assessed both for anti-infectives and for nucleic acid. Higher starch content polyplexes were found to be suitable for high encapsulation efficiency of cationic anti-infectives and preserving their bactericidal function. A cationic carrier was obtained by coating the anionic polyplex with chitosan. Coating allowed for a minimal amount of cationic polymer to be employed and facilitated plasmid DNA loading both within the particle core and on the surface. Transfection studies showed encouraging result, approximately 5% of A549 cells with reporter gene expression. In summary, starch-chitosan complexes are suitable carriers with promising perspectives for pharmaceutical use.

Original languageEnglish
Article number252
Number of pages21
JournalPolymers
Volume10
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Bibliographical note

Publisher Copyright:
© 2018 by the authors.

Keywords

  • Anionic starch
  • Cationic anti-infectives
  • Polymeric nanoparticles
  • Renewable polysaccharides
  • Transfection

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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