Stabilisation and Target Delivery of Nattokinase Using  Compression Coating

Ning Law; Zhibing Zhang

doi:10.1080/03639040601050247

Stabilisation and Target Delivery of Nattokinase Using Compression Coating

Ning Law, Zhibing Zhang

Chemical Engineering

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16 Citations (Scopus)

Abstract

The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase ( NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit (R) L100-55 (EL100-55) and Hydroxypropylcellulose (HPC) by direct compression. The activity of the enzyme was determined using amidolytic assay and its release rates in artificial gastric juice and an intestinal fluid were quantified using bicinchoninic acid assay. Results have shown that the activity of NKCP was pressure independent and the coated tablets protected NKCP from being denatured in the gastric juice, and realized its controlled release to the intestine based on in vitro experiments.

Original language	English
Pages (from-to)	495-503
Number of pages	9
Journal	Drug Development and Industrial Pharmacy
Volume	33
Issue number	5
DOIs	https://doi.org/10.1080/03639040601050247
Publication status	Published - 1 May 2007

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abstract = "The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase ( NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit (R) L100-55 (EL100-55) and Hydroxypropylcellulose (HPC) by direct compression. The activity of the enzyme was determined using amidolytic assay and its release rates in artificial gastric juice and an intestinal fluid were quantified using bicinchoninic acid assay. Results have shown that the activity of NKCP was pressure independent and the coated tablets protected NKCP from being denatured in the gastric juice, and realized its controlled release to the intestine based on in vitro experiments.",

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AB - The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase ( NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit (R) L100-55 (EL100-55) and Hydroxypropylcellulose (HPC) by direct compression. The activity of the enzyme was determined using amidolytic assay and its release rates in artificial gastric juice and an intestinal fluid were quantified using bicinchoninic acid assay. Results have shown that the activity of NKCP was pressure independent and the coated tablets protected NKCP from being denatured in the gastric juice, and realized its controlled release to the intestine based on in vitro experiments.

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JO - Drug Development and Industrial Pharmacy

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Stabilisation and Target Delivery of Nattokinase Using Compression Coating

Abstract

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