Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model

Shu Chen; Gavin K Paterson; Hua Hua Tong; Timothy J Mitchell; Thomas F DeMaria

doi:10.1016/j.femsle.2005.09.052

Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model

Shu Chen, Gavin K Paterson, Hua Hua Tong, Timothy J Mitchell, Thomas F DeMaria

Microbes, Infections and Microbiomes

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Sortase A (SrtA) is required to anchor neuraminidase, beta-galactosidase, and possibly other LPXTG motif proteins to the pneumococcal cell surface. We examined the role of SrtA in Streptococcus pneumoniae nasopharyngeal (NP) colonization in the chinchilla model. The srtA mutant colonized the nasopharynx at a significantly lower level than the D39 parent strain during the second and third week of the carriage, and was eliminated from nasopharynx one week earlier than the D39 pneumococci. Our data indicate that SrtA contributes to pneumococcal NP colonization in this animal model.

Original language	English
Pages (from-to)	151-4
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	253
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.09.052
Publication status	Published - 1 Dec 2005

Keywords

Aminoacyltransferases
Animals
Bacterial Proteins
Cell Membrane
Chinchilla
Cysteine Endopeptidases
Disease Models, Animal
Female
Genes, Bacterial
Humans
Male
Mutation
Nasopharynx
Otitis Media
Phenotype
Pneumococcal Infections
Streptococcus pneumoniae
Time Factors

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10.1016/j.femsle.2005.09.052

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title = "Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model",

abstract = "Sortase A (SrtA) is required to anchor neuraminidase, beta-galactosidase, and possibly other LPXTG motif proteins to the pneumococcal cell surface. We examined the role of SrtA in Streptococcus pneumoniae nasopharyngeal (NP) colonization in the chinchilla model. The srtA mutant colonized the nasopharynx at a significantly lower level than the D39 parent strain during the second and third week of the carriage, and was eliminated from nasopharynx one week earlier than the D39 pneumococci. Our data indicate that SrtA contributes to pneumococcal NP colonization in this animal model.",

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doi = "10.1016/j.femsle.2005.09.052",

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T1 - Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model

AU - Chen, Shu

AU - Paterson, Gavin K

AU - Tong, Hua Hua

AU - Mitchell, Timothy J

AU - DeMaria, Thomas F

PY - 2005/12/1

Y1 - 2005/12/1

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AB - Sortase A (SrtA) is required to anchor neuraminidase, beta-galactosidase, and possibly other LPXTG motif proteins to the pneumococcal cell surface. We examined the role of SrtA in Streptococcus pneumoniae nasopharyngeal (NP) colonization in the chinchilla model. The srtA mutant colonized the nasopharynx at a significantly lower level than the D39 parent strain during the second and third week of the carriage, and was eliminated from nasopharynx one week earlier than the D39 pneumococci. Our data indicate that SrtA contributes to pneumococcal NP colonization in this animal model.

KW - Aminoacyltransferases

KW - Animals

KW - Bacterial Proteins

KW - Cell Membrane

KW - Chinchilla

KW - Cysteine Endopeptidases

KW - Disease Models, Animal

KW - Female

KW - Genes, Bacterial

KW - Humans

KW - Male

KW - Mutation

KW - Nasopharynx

KW - Otitis Media

KW - Phenotype

KW - Pneumococcal Infections

KW - Streptococcus pneumoniae

KW - Time Factors

U2 - 10.1016/j.femsle.2005.09.052

DO - 10.1016/j.femsle.2005.09.052

M3 - Article

C2 - 16246506

SN - 0378-1097

VL - 253

SP - 151

EP - 154

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model

Abstract

Keywords

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