Abstract
Solid-state NMR (ssNMR) is an emerging technique for structural studies of biomolecules, which, unlike solution-state NMR, is applicable to molecules of any size. Significant success was achieved in structure determination of membrane proteins, amyloid fibrils, and large protein complexes in the past decade. Conversely, only a small number of studies have so far addressed RNA or protein-RNA complexes. Here, we discuss current progresses in the application of ssNMR to RNA structural studies. We review sample preparation and resonance assignment strategies and discuss the structural information that could be obtained so far for RNA by ssNMR.
Original language | English |
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Pages (from-to) | 119-128 |
Number of pages | 10 |
Journal | eMagRes |
Volume | 3 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2014 |
Bibliographical note
Publisher Copyright:© 2014 John Wiley & Sons, Ltd.
Keywords
- resonance assignment
- RNA
- sample preparation
- solid-state NMR
- structural information
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Biomedical Engineering
- Radiology Nuclear Medicine and imaging
- Spectroscopy