Software package for off-Axis digital holographic microscopy imaging processing

Tienan Xu; Xuefei He; Zhiduo Zhang; Samantha Montague; Elizabeth Gardiner; Woie Ming Lee

doi:10.1117/12.2539541

Software package for off-Axis digital holographic microscopy imaging processing

Tienan Xu^*, Xuefei He, Zhiduo Zhang, Samantha Montague, Elizabeth Gardiner, Woie Ming Lee

^*Corresponding author for this work

Cardiovascular Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-Axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool's usability in biomedical research. In this paper, a software package is developed for off-Axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-Axis DHM setups, aiming to increase the efficiency of DHM's bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.

Original language	English
Title of host publication	Biophotonics Australasia 2019
Editors	Ewa M. Goldys, Brant C. Gibson
Publisher	SPIE
ISBN (Electronic)	9781510631441
DOIs	https://doi.org/10.1117/12.2539541
Publication status	Published - 2019
Event	Biophotonics Australasia 2019 - Melbourne, Australia Duration: 9 Dec 2019 → 12 Dec 2019

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11202
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biophotonics Australasia 2019
Country/Territory	Australia
City	Melbourne
Period	9/12/19 → 12/12/19

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.2539541

Cite this

Xu, T., He, X., Zhang, Z., Montague, S., Gardiner, E., & Lee, W. M. (2019). Software package for off-Axis digital holographic microscopy imaging processing. In E. M. Goldys, & B. C. Gibson (Eds.), Biophotonics Australasia 2019 Article 112021C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11202). SPIE. https://doi.org/10.1117/12.2539541

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title = "Software package for off-Axis digital holographic microscopy imaging processing",

abstract = "Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-Axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool's usability in biomedical research. In this paper, a software package is developed for off-Axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-Axis DHM setups, aiming to increase the efficiency of DHM's bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.",

author = "Tienan Xu and Xuefei He and Zhiduo Zhang and Samantha Montague and Elizabeth Gardiner and Lee, {Woie Ming}",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Biophotonics Australasia 2019 ; Conference date: 09-12-2019 Through 12-12-2019",

year = "2019",

doi = "10.1117/12.2539541",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

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editor = "Goldys, {Ewa M.} and Gibson, {Brant C.}",

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Xu, T, He, X, Zhang, Z, Montague, S, Gardiner, E & Lee, WM 2019, Software package for off-Axis digital holographic microscopy imaging processing. in EM Goldys & BC Gibson (eds), Biophotonics Australasia 2019., 112021C, Proceedings of SPIE - The International Society for Optical Engineering, vol. 11202, SPIE, Biophotonics Australasia 2019, Melbourne, Australia, 9/12/19. https://doi.org/10.1117/12.2539541

Software package for off-Axis digital holographic microscopy imaging processing. / Xu, Tienan; He, Xuefei; Zhang, Zhiduo et al.
Biophotonics Australasia 2019. ed. / Ewa M. Goldys; Brant C. Gibson. SPIE, 2019. 112021C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11202).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - He, Xuefei

AU - Zhang, Zhiduo

AU - Montague, Samantha

AU - Gardiner, Elizabeth

AU - Lee, Woie Ming

PY - 2019

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AB - Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-Axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool's usability in biomedical research. In this paper, a software package is developed for off-Axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-Axis DHM setups, aiming to increase the efficiency of DHM's bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.

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AN - SCOPUS:85079889924

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biophotonics Australasia 2019

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Software package for off-Axis digital holographic microscopy imaging processing

Abstract

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Conference

Bibliographical note

ASJC Scopus subject areas

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Cite this