Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

Jean-Louis H A Duprey, Gemma A Bullen, Zheng-Yun Zhao, Dario Maria Bassani, Anna F A Peacock, John Wilkie, James H R Tucker

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
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Abstract

The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

Original languageEnglish
JournalACS chemical biology
Early online date18 Nov 2015
DOIs
Publication statusPublished - 2016

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