Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

Jean-Louis H A Duprey; Gemma A Bullen; Zheng-Yun Zhao; Dario Maria Bassani; Anna F A Peacock; John Wilkie; James H R Tucker

doi:10.1021/acschembio.5b00796

Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

Jean-Louis H A Duprey, Gemma A Bullen, Zheng-Yun Zhao, Dario Maria Bassani, Anna F A Peacock, John Wilkie, James H R Tucker

Research output: Contribution to journal › Article › peer-review

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Abstract

The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

Original language	English
Journal	ACS chemical biology
Early online date	18 Nov 2015
DOIs	https://doi.org/10.1021/acschembio.5b00796
Publication status	Published - 2016

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10.1021/acschembio.5b00796

Duprey_et_al_Single_Site_Discrimination_ACS_Chemical_Biology_Post-Print
Final Version of Record published as: Duprey, Jean-Louis HA, et al. "Single Site Discrimination of Cytosine, 5-Methylcytosine, and 5-Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes." ACS Chemical Biology (2015). Available at: http://dx.doi.org/10.1021/acschembio.5b00796 Checked Jan 2016
Accepted author manuscript, 718 KBLicence: None: All rights reserved

Functional DNA-based assemblies
Tucker, J. (Principal Investigator)
Engineering & Physical Science Research Council
16/03/09 → 31/03/14
Project: Research Councils

Cite this

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title = "Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes",

abstract = "The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.",

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language = "English",

journal = "ACS chemical biology",

issn = "1554-8929",

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AU - Duprey, Jean-Louis H A

AU - Bullen, Gemma A

AU - Zhao, Zheng-Yun

AU - Bassani, Dario Maria

AU - Peacock, Anna F A

AU - Wilkie, John

AU - Tucker, James H R

PY - 2016

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N2 - The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

AB - The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

U2 - 10.1021/acschembio.5b00796

DO - 10.1021/acschembio.5b00796

M3 - Article

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SN - 1554-8929

JO - ACS chemical biology

JF - ACS chemical biology

ER -

Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

Abstract

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Functional DNA-based assemblies

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