Single molecule localisation and structured illumination microscopy of platelet proteins

Natalie Poulter; Abdullah Khan; Chiara Pallini; Steven Thomas

doi:10.1007/978-1-4939-8585-2

Single molecule localisation and structured illumination microscopy of platelet proteins

Natalie Poulter, Abdullah Khan, Chiara Pallini, Steven Thomas

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Abstract

Super-resolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Super-resolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, Structured Illumination Microscopy (SIM) and Single Molecule Localisation Microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialisation by the main microscope manufacturers. This chapter will provide background to the two techniques, practical considerations for their use and protocols for their application to platelet biology.

Original language	English
Title of host publication	Platelets and megakaryocytes
Subtitle of host publication	volume 4, advanced protocols and perspectives
Publisher	Humana Press
Chapter	3
Volume	4
ISBN (Print)	978-1-4939-8584-5
DOIs	https://doi.org/10.1007/978-1-4939-8585-2
Publication status	Published - 1 Sept 2018

Publication series

Name	Methods in Molecular Biology
Volume	1812
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Platelets
Super-resolution
Single Molecule Localisation Microscopy
SMLM
Direct Stochastic Optical Reconstruction Microscopy
dSTORM
Structured Illumination Microscopy
SIM

Access to Document

10.1007/978-1-4939-8585-2

Poulter_et_al_MIMB_chapter
Final Version of Record published as detailed above. Checked 7.9.18
Accepted author manuscript, 240 KBLicence: None: All rights reserved
STORM figure A5 11-5-17Accepted author manuscript, 3.28 MBLicence: None: All rights reserved
SIM figure A5 11-05-2017Accepted author manuscript, 6.57 MBLicence: None: All rights reserved
Drift figure 16-5-17Accepted author manuscript, 1.03 MBLicence: None: All rights reserved

Cite this

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title = "Single molecule localisation and structured illumination microscopy of platelet proteins",

abstract = "Super-resolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Super-resolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, Structured Illumination Microscopy (SIM) and Single Molecule Localisation Microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialisation by the main microscope manufacturers. This chapter will provide background to the two techniques, practical considerations for their use and protocols for their application to platelet biology.",

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T1 - Single molecule localisation and structured illumination microscopy of platelet proteins

AU - Poulter, Natalie

AU - Khan, Abdullah

AU - Pallini, Chiara

AU - Thomas, Steven

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Y1 - 2018/9/1

N2 - Super-resolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Super-resolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, Structured Illumination Microscopy (SIM) and Single Molecule Localisation Microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialisation by the main microscope manufacturers. This chapter will provide background to the two techniques, practical considerations for their use and protocols for their application to platelet biology.

AB - Super-resolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Super-resolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, Structured Illumination Microscopy (SIM) and Single Molecule Localisation Microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialisation by the main microscope manufacturers. This chapter will provide background to the two techniques, practical considerations for their use and protocols for their application to platelet biology.

KW - Platelets

KW - Super-resolution

KW - Single Molecule Localisation Microscopy

KW - SMLM

KW - Direct Stochastic Optical Reconstruction Microscopy

KW - dSTORM

KW - Structured Illumination Microscopy

KW - SIM

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DO - 10.1007/978-1-4939-8585-2

M3 - Chapter

SN - 978-1-4939-8584-5

VL - 4

T3 - Methods in Molecular Biology

BT - Platelets and megakaryocytes

PB - Humana Press

ER -

Single molecule localisation and structured illumination microscopy of platelet proteins

Abstract

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Keywords

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