Super-resolution microscopy has become increasingly widespread over the past 5 years and allows users to image biological processes below the diffraction limit of traditional fluorescence microscopy where resolution is restricted to approximately 250 nm. Super-resolution refers to a wide range of techniques which employ different approaches to circumvent the diffraction limit. Two of these approaches, Structured Illumination Microscopy (SIM) and Single Molecule Localisation Microscopy (SMLM), which provide a doubling and tenfold increase in resolution respectively, are dominating the field. This is partly because of the insights into biology they offer and partly because of their commercialisation by the main microscope manufacturers. This chapter will provide background to the two techniques, practical considerations for their use and protocols for their application to platelet biology.
|Title of host publication||Platelets and megakaryocytes|
|Subtitle of host publication||volume 4, advanced protocols and perspectives|
|Publication status||Published - 1 Sep 2018|
|Name||Methods in Molecular Biology|
- Single Molecule Localisation Microscopy
- Direct Stochastic Optical Reconstruction Microscopy
- Structured Illumination Microscopy