Simultaneous orthogonal dual-click approach to tough, in-situ-forming hydrogels for cell encapsulation

Vinh X. Truong, Matthew P. Ablett, Stephen M. Richardson, Judith A. Hoyland, Andrew P. Dove

Research output: Contribution to journalArticlepeer-review

143 Citations (Scopus)


The use of tough hydrogels as biomaterials is limited as a consequence of time-consuming fabrication techniques, toxic starting materials, and large strain hysteresis under deformation. Herein, we report the simultaneous application of nucleophilic thiol-yne and inverse electron-demand Diels-Alder additions to independently create two interpenetrating networks in a simple one-step procedure. The resultant hydrogels display compressive stresses of 14-15 MPa at 98% compression without fracture or hysteresis upon repeated load. The hydrogel networks can be spatially and temporally postfunctionalized via radical thiylation and/or inverse electron-demand Diels-Alder addition to residual functional groups within the network. Furthermore, gelation occurs rapidly under physiological conditions, enabling encapsulation of human cells.

Original languageEnglish
Pages (from-to)1618-22
Number of pages5
JournalJournal of the American Chemical Society
Issue number4
Early online date15 Jan 2015
Publication statusPublished - 26 Jan 2015


  • Biocompatible Materials/chemistry
  • Cells, Immobilized/cytology
  • Click Chemistry
  • Cycloaddition Reaction
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry
  • Mesenchymal Stromal Cells/cytology
  • Models, Molecular
  • Polyethylene Glycols/chemistry
  • Stress, Mechanical
  • Sulfhydryl Compounds/chemistry
  • Tissue Engineering


Dive into the research topics of 'Simultaneous orthogonal dual-click approach to tough, in-situ-forming hydrogels for cell encapsulation'. Together they form a unique fingerprint.

Cite this