Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives

E Sánchez-Morán, E Benavente, J Orellana

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Multicolour genomic in situ hybridization was carried out in wheat-rye hybrids and in a wheat-rye translocation line. Different hybridization conditions and mixture compositions were used, and A, B and D genomes of hexaploid wheat as well as the R genome of rye were distinguished simultaneously in somatic cells. Combination of genomic and rDNA probes in multicolour in situ hybridization was also performed to identify chromosomes within a specific genome.

Original languageEnglish
Pages (from-to)249-52
Number of pages4
JournalHeredity
Volume83 ( Pt 3)
Publication statusPublished - Sep 1999

Keywords

  • DNA, Plant
  • Genome, Plant
  • In Situ Hybridization, Fluorescence
  • Secale
  • Triticum

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