Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells

Ben Scheven, Jane Millard, Paul Cooper, Simon Lea, Anthony Walmsley, Anthony Smith

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In this study, the effects of low frequency ultrasound (US) were examined on odontoblasts, the primary cell responsible for dentinogenesis and dentine repair. An established odontoblast-like cell line, MDPC-23, was subjected to 30 kHz ultrasound at three different power settings. US induced a marginal level of cell death (3% to 4%) at lower amplitudes rising to 25% cell death at the highest power tested. The latter was reflected in a 30% decrease in cell attachment after 4 to 24 h of culture, while the number of adherent cells was reduced by approximately 10% to 15% in the lower power groups. Cell replication after 24 h, as measured by BrdU incorporation, showed no significant changes in the US-treated groups. Gene expression analyses demonstrated a moderate dose-dependent increase in the expression of GAPDH (glyseraldehyde-3-phosphate dehydrogenase)-normalised collagen type I, osteopontin (OPN), transforming growth factor-beta1 (TGFbeta1) and the heat shock protein (hsp) 70. The greatest change was found in the expression of the small hsp 25/27, which showed a two- to six-fold increase following US treatment. No significant effects were observed for alkaline phosphatase (ALP) and core-binding factor A1 (CBFA1/Runx2) expression levels. This is the first report describing US effects on odontoblasts. Further studies are warranted to elucidate US effects on odontoblast function and to evaluate US as a therapeutic application in dentine repair.
Original languageEnglish
Pages (from-to)1475-82
Number of pages8
JournalUltrasound in Medicine & Biology
Volume33
Issue number9
DOIs
Publication statusPublished - 1 Sep 2007

Keywords

  • cell proliferation
  • cell viability
  • odontoblast
  • ultrasound
  • gene expression
  • cell adhesion
  • heat shock protein
  • tooth repair

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