Severe Zinc Depletion of Escherichia coli ROLES FOR HIGH AFFINITY ZINC BINDING BY ZinT, ZINC TRANSPORT AND ZINC-INDEPENDENT PROTEINS

AI Graham, S Hunt, SL Stokes, N Bramall, Josephine Bunch, AG Cox, CW McLeod, RK Poole

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Zinc ions play indispensable roles in biological chemistry. However, bacteria have an impressive ability to acquire Zn2+ from the environment, making it exceptionally difficult to achieve Zn2+ deficiency, and so a comprehensive understanding of the importance of Zn2+ has not been attained. Reduction of the Zn2+ content of Escherichia coli growth medium to 60 nM or less is reported here for the first time, without recourse to chelators of poor specificity. Cells grown in Zn2+-deficient medium had a reduced growth rate and contained up to five times less cellular Zn2+. To understand global responses to Zn2+ deficiency, microarray analysis was conducted of cells grown under Zn2+-replete and Zn2+-depleted conditions in chemostat cultures. Nine genes were up-regulated more than 2-fold (p
Original languageEnglish
Pages (from-to)18377-18389
Number of pages13
JournalJournal of Biological Chemistry
Volume284
Issue number27
DOIs
Publication statusPublished - 1 Jul 2009

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