Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent

Sonia Molina; V Castet; L Pichard-Garcia; C Wychowski; E Meurs; JM Pascussi; C Sureau; JM Fabre; A SaCunha; D Larrey; J Dubuisson; J Coste; Jane McKeating; P Maurel; C Fournier-Wirth

doi:10.1128/JVI.01443-07

Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent

Sonia Molina, V Castet, L Pichard-Garcia, C Wychowski, E Meurs, JM Pascussi, C Sureau, JM Fabre, A SaCunha, D Larrey, J Dubuisson, J Coste, Jane McKeating, P Maurel, C Fournier-Wirth

Immunology and Immunotherapy

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Abstract

Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

Original language	English
Pages (from-to)	569-574
Number of pages	6
Journal	Journal of virology
Volume	82
Issue number	1
Early online date	17 Oct 2007
DOIs	https://doi.org/10.1128/JVI.01443-07
Publication status	Published - 17 Oct 2007

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10.1128/JVI.01443-07

Mechanisms of Hepatitis C Virus Induced Hepatocyte Injury
McKeating, J. (Principal Investigator) & Balfe, P. (Co-Investigator)
Medical Research Council
1/10/09 → 30/09/12
Project: Research Councils

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Molina, S., Castet, V., Pichard-Garcia, L., Wychowski, C., Meurs, E., Pascussi, JM., Sureau, C., Fabre, JM., SaCunha, A., Larrey, D., Dubuisson, J., Coste, J., McKeating, J., Maurel, P., & Fournier-Wirth, C. (2007). Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent. Journal of virology, 82(1), 569-574. https://doi.org/10.1128/JVI.01443-07

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title = "Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent",

abstract = "Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.",

author = "Sonia Molina and V Castet and L Pichard-Garcia and C Wychowski and E Meurs and JM Pascussi and C Sureau and JM Fabre and A SaCunha and D Larrey and J Dubuisson and J Coste and Jane McKeating and P Maurel and C Fournier-Wirth",

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Molina, S, Castet, V, Pichard-Garcia, L, Wychowski, C, Meurs, E, Pascussi, JM, Sureau, C, Fabre, JM, SaCunha, A, Larrey, D, Dubuisson, J, Coste, J, McKeating, J, Maurel, P & Fournier-Wirth, C 2007, 'Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent', Journal of virology, vol. 82, no. 1, pp. 569-574. https://doi.org/10.1128/JVI.01443-07

TY - JOUR

T1 - Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent

AU - Molina, Sonia

AU - Castet, V

AU - Pichard-Garcia, L

AU - Wychowski, C

AU - Meurs, E

AU - Pascussi, JM

AU - Sureau, C

AU - Fabre, JM

AU - SaCunha, A

AU - Larrey, D

AU - Dubuisson, J

AU - Coste, J

AU - McKeating, Jane

AU - Maurel, P

AU - Fournier-Wirth, C

PY - 2007/10/17

Y1 - 2007/10/17

N2 - Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

AB - Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

U2 - 10.1128/JVI.01443-07

DO - 10.1128/JVI.01443-07

M3 - Article

C2 - 17942559

SN - 1098-5514

VL - 82

SP - 569

EP - 574

JO - Journal of virology

JF - Journal of virology

IS - 1

ER -

Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent

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Mechanisms of Hepatitis C Virus Induced Hepatocyte Injury

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