Sequence of plasmid pBS228 and reconstruction of the IncP-1α phylogeny

Anthony Haines, Karen Jones, Sarah Batt, I Kosheleva, Christopher Thomas

Research output: Contribution to journalArticle

36 Citations (Scopus)


The antibiotic resistance plasmid pBS228 has been completely sequenced, and revealed to be descended from a plasmid virtually identical to the Birmingham IncP-1alpha plasmid RK2/RP4/RP1. However, it has three additional transposon insertions, one of which is responsible for the extra antibiotic resistances conferred. Loss of kanamycin resistance, which is characteristic of most IncP-1alpha plasmids, is the result of this insertion. A second transposon causes inactivation of the mating pair formation apparatus, rendering the plasmid non-self-transmissible. Comparison with the published data for other IncP-1alpha plasmids gives insight into the recent evolutionary history of this group as well as the acquisition and transmission of one of the first ampicillin resistance transposons discovered.
Original languageEnglish
Pages (from-to)76-83
Number of pages8
Issue number1
Publication statusPublished - 1 Jul 2007


  • transposable element
  • antibiotic resistance
  • broad host range
  • ampicillin resistance
  • evolution
  • Tn1


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