Septin-mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae

Yasin F Dagdas, Kae Yoshino, Gulay Dagdas, Lauren S Ryder, Ewa Bielska, Gero Steinberg, Nicholas J Talbot

Research output: Contribution to journalArticlepeer-review

182 Citations (Scopus)


To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures the leaf cuticle to gain entry to plant tissue. Here, we report that a toroidal F-actin network assembles in the appressorium by means of four septin guanosine triphosphatases, which polymerize into a dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via the ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at the appressorium plasma membrane. The septin ring assembles in a Cdc42- and Chm1-dependent manner and forms a diffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Aldrich syndrome protein Las17 at the point of penetration. Septins thereby provide the cortical rigidity and membrane curvature necessary for protrusion of a rigid penetration peg to breach the leaf surface.

Original languageEnglish
Pages (from-to)1590-5
Number of pages6
Issue number6088
Publication statusPublished - 22 Jun 2012


  • Actin Cytoskeleton
  • Actins
  • Cell Membrane
  • Diffusion
  • Fungal Proteins
  • Magnaporthe
  • Microfilament Proteins
  • Mutation
  • Oryza
  • Phosphatidylinositols
  • Plant Diseases
  • Plant Leaves
  • Protein Interaction Domains and Motifs
  • Recombinant Fusion Proteins
  • Septins
  • cdc42 GTP-Binding Protein


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