Separation of cis and trans isomers of polyproline by FAIMS mass spectrometry

Andrew Creese, Helen Cooper

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
159 Downloads (Pure)


High field asymmetric waveform ion mobility spectrometry (FAIMS) is well-established as a tool for separating peptide isomers (sequence inversions and post-translationally modified localization variants). Here, we demonstrate the FAIMS is able to differentiate cis and trans isomers of polyproline. Polyproline assumes an all-cis conformation—the PPI helix—in 1-propanol, and an all-trans conformation—the PPII helix—in aqueous solutions. Differentiation of these conformers may be achieved both through use of a cylindrical FAIMS device and a miniaturized ultrahigh field planar FAIMS device.
Original languageEnglish
Pages (from-to)2071–2074
JournalJournal of the American Society for Mass Spectrometry
Issue number12
Early online date4 Oct 2016
Publication statusPublished - Dec 2016


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