Separase, securin and Rad21 in neural cell growth

Helen Pemberton, Jayne Franklyn, Kristien Boelaert, Shiao-yng Chan, Dae Kim, C Kim, SY Cheng, Mark Kilby, Christopher McCabe

Research output: Contribution to journalArticle

10 Citations (Scopus)


The key mitotic regulator securin is expressed at low levels in fetal brain compared with adult, and modulates the proliferation of human embryonic neuronal N-Tera2 (NT2) cells. We now examine the function and expression of securin's interacting partner separase, along with Rad21, the functional component of cohesin, which is cleaved by separase following interaction with securin. In contrast to securin, the cleaved forms of separase and Rad21 were highly expressed in human fetal cerebral cortex compared with adult. In a murine model of absent securin expression - the PTTG knock-out mouse - separase and Rad21 were over-expressed in multiple brain regions. In addition, cDNA array analysis of other key mitotic regulators additionally identified cyclin C and sestrin 2 to be induced in the brains of securin-null mice compared with wild type. Further, Rad21 mRNA expression was highly correlated with that of securin, separase, cyclin C and sestrin 2 in fetal brains. In embryonic neuronal NT2 cells, siRNA repression of separase failed to significantly alter cell turnover, whereas repression of securin expression resulted in increased levels of the activated forms of Rad21 and separase, and promoted cell proliferation. Our data suggest that the co-ordinated expression of separase, securin and Rad21 is fundamental for the developing brain.
Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalJournal of Cellular Physiology
Issue number1
Early online date1 Jan 2007
Publication statusPublished - 1 Oct 2007


Dive into the research topics of 'Separase, securin and Rad21 in neural cell growth'. Together they form a unique fingerprint.

Cite this