Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples

Gabriella L Morley; Stephen Taylor; Sian Jossi; Marisol Perez-Toledo; Sian E Faustini; Edith Marcial-Juarez; Adrian M Shields; Margaret Goodall; Joel D Allen; Yasunori Watanabe; Maddy L Newby; Max Crispin; Mark T Drayson; Adam F Cunningham; Alex G Richter; Matthew K O'Shea

doi:10.3201/eid2612.203309

Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples

Gabriella L Morley
, Stephen Taylor
, Sian Jossi
, Marisol Perez-Toledo
, Sian E Faustini
, Edith Marcial-Juarez
, Adrian M Shields
, Margaret Goodall
, Joel D Allen
, Yasunori Watanabe
, Maddy L Newby
, Max Crispin
, Mark T Drayson
, Adam F Cunningham
, Alex G Richter
, Matthew K O'Shea

Research output: Contribution to journal › Article › peer-review

Abstract

Dried blood spot (DBS) samples can be used for the detection of severe acute respiratory syndrome coronavirus 2 spike antibodies. DBS sampling is comparable to matched serum samples with a relative 98.1% sensitivity and 100% specificity. Thus, DBS sampling offers an alternative for population-wide serologic testing in the coronavirus pandemic.

Original language	English
Pages (from-to)	2970-2973
Number of pages	4
Journal	Emerging infectious diseases
Volume	26
Issue number	12
DOIs	https://doi.org/10.3201/eid2612.203309
Publication status	Published - Dec 2020

Keywords

Antibodies, Viral/immunology
COVID-19/diagnosis
COVID-19 Serological Testing/methods
Case-Control Studies
Dried Blood Spot Testing/economics
Humans
Predictive Value of Tests
SARS-CoV-2/immunology
Spike Glycoprotein, Coronavirus/immunology

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10.3201/eid2612.203309

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Morley, G. L., Taylor, S., Jossi, S., Perez-Toledo, M., Faustini, S. E., Marcial-Juarez, E., Shields, A. M., Goodall, M., Allen, J. D., Watanabe, Y., Newby, M. L., Crispin, M., Drayson, M. T., Cunningham, A. F., Richter, A. G., & O'Shea, M. K. (2020). Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples. Emerging infectious diseases, 26(12), 2970-2973. https://doi.org/10.3201/eid2612.203309

@article{56aa606c58c5400b8f82e8ec23fca65e,

title = "Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples",

abstract = "Dried blood spot (DBS) samples can be used for the detection of severe acute respiratory syndrome coronavirus 2 spike antibodies. DBS sampling is comparable to matched serum samples with a relative 98.1\% sensitivity and 100\% specificity. Thus, DBS sampling offers an alternative for population-wide serologic testing in the coronavirus pandemic.",

keywords = "Antibodies, Viral/immunology, COVID-19/diagnosis, COVID-19 Serological Testing/methods, Case-Control Studies, Dried Blood Spot Testing/economics, Humans, Predictive Value of Tests, SARS-CoV-2/immunology, Spike Glycoprotein, Coronavirus/immunology",

author = "Morley, \{Gabriella L\} and Stephen Taylor and Sian Jossi and Marisol Perez-Toledo and Faustini, \{Sian E\} and Edith Marcial-Juarez and Shields, \{Adrian M\} and Margaret Goodall and Allen, \{Joel D\} and Yasunori Watanabe and Newby, \{Maddy L\} and Max Crispin and Drayson, \{Mark T\} and Cunningham, \{Adam F\} and Richter, \{Alex G\} and O'Shea, \{Matthew K\}",

year = "2020",

month = dec,

doi = "10.3201/eid2612.203309",

language = "English",

volume = "26",

pages = "2970--2973",

journal = "Emerging infectious diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention (CDC)",

number = "12",

}

Morley, GL, Taylor, S, Jossi, S, Perez-Toledo, M , Faustini, SE , Marcial-Juarez, E , Shields, AM, Goodall, M, Allen, JD, Watanabe, Y, Newby, ML, Crispin, M, Drayson, MT , Cunningham, AF , Richter, AG & O'Shea, MK 2020, 'Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples', Emerging infectious diseases, vol. 26, no. 12, pp. 2970-2973. https://doi.org/10.3201/eid2612.203309

TY - JOUR

T1 - Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples

AU - Morley, Gabriella L

AU - Taylor, Stephen

AU - Jossi, Sian

AU - Perez-Toledo, Marisol

AU - Faustini, Sian E

AU - Marcial-Juarez, Edith

AU - Shields, Adrian M

AU - Goodall, Margaret

AU - Allen, Joel D

AU - Watanabe, Yasunori

AU - Newby, Maddy L

AU - Crispin, Max

AU - Drayson, Mark T

AU - Cunningham, Adam F

AU - Richter, Alex G

AU - O'Shea, Matthew K

PY - 2020/12

Y1 - 2020/12

N2 - Dried blood spot (DBS) samples can be used for the detection of severe acute respiratory syndrome coronavirus 2 spike antibodies. DBS sampling is comparable to matched serum samples with a relative 98.1% sensitivity and 100% specificity. Thus, DBS sampling offers an alternative for population-wide serologic testing in the coronavirus pandemic.

AB - Dried blood spot (DBS) samples can be used for the detection of severe acute respiratory syndrome coronavirus 2 spike antibodies. DBS sampling is comparable to matched serum samples with a relative 98.1% sensitivity and 100% specificity. Thus, DBS sampling offers an alternative for population-wide serologic testing in the coronavirus pandemic.

KW - Antibodies, Viral/immunology

KW - COVID-19/diagnosis

KW - COVID-19 Serological Testing/methods

KW - Case-Control Studies

KW - Dried Blood Spot Testing/economics

KW - Humans

KW - Predictive Value of Tests

KW - SARS-CoV-2/immunology

KW - Spike Glycoprotein, Coronavirus/immunology

UR - http://dx.doi.org/10.3201/eid2612.203309

U2 - 10.3201/eid2612.203309

DO - 10.3201/eid2612.203309

M3 - Article

C2 - 32969788

SN - 1080-6040

VL - 26

SP - 2970

EP - 2973

JO - Emerging infectious diseases

JF - Emerging infectious diseases

IS - 12

ER -

Sensitive Detection of SARS-CoV-2-Specific Antibodies in Dried Blood Spot Samples

Abstract

Keywords

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