Secondary structure and 1H, 13C and 15N resonance assignments of BamE, a component of the outer membrane protein assembly machinery in Escherichia coli.

Timothy Knowles; Pooja Sridhar; Sandya Rajesh; Eleni Manoli; Michael Overduin; Ian Henderson

doi:10.1007/s12104-010-9236-7

Secondary structure and 1H, 13C and 15N resonance assignments of BamE, a component of the outer membrane protein assembly machinery in Escherichia coli.

Timothy Knowles, Pooja Sridhar, Sandya Rajesh, Eleni Manoli, Michael Overduin, Ian Henderson

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

We report the (1)H, (13)C and (15)N backbone and side chain chemical shift assignments and secondary structure of the Escherichia coli protein BamE, a subunit of the BAM (Omp85) complex, the β-barrel assembly machinery present in all Gram-negative bacteria, mitochondria and chloroplasts and is essential for viability.

Original language	English
Pages (from-to)	179-81
Number of pages	3
Journal	Biomolecular NMR Assignments
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/s12104-010-9236-7
Publication status	Published - 1 Oct 2010

Keywords

BAM
Omp85
SmpA
Backbone resonance assignment
YaeT
Secondary structure
BamE
NMR

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10.1007/s12104-010-9236-7

Structural Basis of the Outer Membrane Protein Assembly System by NMR Spectroscopy
Overduin, M. (Principal Investigator), Henderson, I. (Co-Investigator) & Knowles, T. (Co-Investigator)
Biotechnology & Biological Sciences Research Council
1/12/09 → 30/11/13
Project: Research Councils
Understanding the Role of the Bam Complex in the Biogenesis of Outer Membrane Proteins
Henderson, I. (Principal Investigator) & Overduin, M. (Co-Investigator)
Medical Research Council
1/01/09 → 31/12/11
Project: Research Councils
POTRA Domain Structure and Function by NMR Spectroscopy
Overduin, M. (Principal Investigator) & Henderson, I. (Co-Investigator)
Biotechnology & Biological Sciences Research Council
1/10/07 → 30/09/10
Project: Research Councils

Cite this

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title = "Secondary structure and 1H, 13C and 15N resonance assignments of BamE, a component of the outer membrane protein assembly machinery in Escherichia coli.",

abstract = "We report the (1)H, (13)C and (15)N backbone and side chain chemical shift assignments and secondary structure of the Escherichia coli protein BamE, a subunit of the BAM (Omp85) complex, the β-barrel assembly machinery present in all Gram-negative bacteria, mitochondria and chloroplasts and is essential for viability.",

keywords = "BAM, Omp85, SmpA, Backbone resonance assignment, YaeT, Secondary structure, BamE, NMR",

author = "Timothy Knowles and Pooja Sridhar and Sandya Rajesh and Eleni Manoli and Michael Overduin and Ian Henderson",

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AU - Knowles, Timothy

AU - Sridhar, Pooja

AU - Rajesh, Sandya

AU - Manoli, Eleni

AU - Overduin, Michael

AU - Henderson, Ian

PY - 2010/10/1

Y1 - 2010/10/1

N2 - We report the (1)H, (13)C and (15)N backbone and side chain chemical shift assignments and secondary structure of the Escherichia coli protein BamE, a subunit of the BAM (Omp85) complex, the β-barrel assembly machinery present in all Gram-negative bacteria, mitochondria and chloroplasts and is essential for viability.

AB - We report the (1)H, (13)C and (15)N backbone and side chain chemical shift assignments and secondary structure of the Escherichia coli protein BamE, a subunit of the BAM (Omp85) complex, the β-barrel assembly machinery present in all Gram-negative bacteria, mitochondria and chloroplasts and is essential for viability.

KW - BAM

KW - Omp85

KW - SmpA

KW - Backbone resonance assignment

KW - YaeT

KW - Secondary structure

KW - BamE

KW - NMR

U2 - 10.1007/s12104-010-9236-7

DO - 10.1007/s12104-010-9236-7

M3 - Article

C2 - 20526702

VL - 4

SP - 179

EP - 181

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 2

ER -

Secondary structure and 1H, 13C and 15N resonance assignments of BamE, a component of the outer membrane protein assembly machinery in Escherichia coli.

Abstract

Keywords

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Projects

Structural Basis of the Outer Membrane Protein Assembly System by NMR Spectroscopy

Understanding the Role of the Bam Complex in the Biogenesis of Outer Membrane Proteins

POTRA Domain Structure and Function by NMR Spectroscopy

Cite this