Searching for telomeric sequences in two Allium species

N Cuñado, E Sánchez-Morán, J Barrios, J L Santos

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Some Alliaceae species have no tandemly repeated TTTAGGG sequences. Instead, at the very end of their chromosomes, there are highly repetitive satellite and (or) rDNA sequences. These sequences apparently replace the canonical plant telomeric sequences in these species. A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs), combined with fluorescent in situ hybridization, has revealed that telomeric chromatin is tightly condensed at the ends of SCs in plants and animals. Using this method, we have tested the organization and location of those sequences postulated to cap the chromosomes in two species of the genus Allium: A. cepa and A. altaicum. We have also extended this study to other putative telomere candidates, such as LTR (long terminal repeat) and non-LTR retrotransposons. None of the DNA sequences analyzed showed the characteristic telomeric organization at pachytene.

Original languageEnglish
Pages (from-to)640-3
Number of pages4
Issue number4
Publication statusPublished - Aug 2001


  • Allium
  • Animals
  • Chromosomes
  • Drosophila melanogaster
  • In Situ Hybridization, Fluorescence
  • Microsatellite Repeats
  • Species Specificity
  • Telomere
  • Terminal Repeat Sequences


Dive into the research topics of 'Searching for telomeric sequences in two Allium species'. Together they form a unique fingerprint.

Cite this