Role for lysosomal phospholipase A2 in iNKT cell-mediated CD1d recognition

Crina Paduraru, Jelena S Bezbradica, Amit Kunte, Robert Kelly, James A Shayman, Natacha Veerapen, Liam R Cox, Gurdyal S Besra, Peter Cresswell

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Invariant natural killer T (iNKT) cells recognize self lipid antigens presented by CD1d molecules. The nature of the self-antigens involved in the development and maturation of iNKT cells is poorly defined. Lysophospholipids are self-antigens presented by CD1d that are generated through the action of phospholipases A1 and A2. Lysosomal phospholipase A2 (LPLA2, group XV phospholipase A2) resides in the endocytic system, the main site where CD1d antigen acquisition occurs, suggesting that it could be particularly important in CD1d function. We find that Lpla2(-/-) mice show a decrease in iNKT cell numbers that is neither the result of a general effect on the development of lymphocyte populations nor of effects on CD1d expression. However, endogenous lipid antigen presentation by CD1d is reduced in the absence of LPLA2. Our data suggest that LPLA2 plays a role in the generation of CD1d complexes with thymic lipids required for the normal selection and maturation of iNKT cells.
Original languageEnglish
Pages (from-to)5097-102
Number of pages6
JournalNational Academy of Sciences. Proceedings
Issue number13
Publication statusPublished - 26 Mar 2013


  • Acyltransferases
  • Animals
  • Antigen Presentation
  • Antigens
  • Antigens, CD1d
  • Lymphocyte Count
  • Lysosomes
  • Mice
  • Mice, Knockout
  • Natural Killer T-Cells
  • Phospholipases A2
  • Thymus Gland


Dive into the research topics of 'Role for lysosomal phospholipase A2 in iNKT cell-mediated CD1d recognition'. Together they form a unique fingerprint.

Cite this