TY - JOUR
T1 - Rhodotorulic acid production by Rhodotorula mucilaginosa
AU - Andersen, D
AU - Renshaw, Joanna
AU - Wiebe, MG
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Rhodotorula mucilaginosa produces the siderophore rhodotorulic acid (RA) when grown in iron-limited conditions. R. mucilaginosa grew at rates between 0.10 and 0.19 h(-1) in iron-restricted conditions, depending on the carbon source, and at 0.23 h(-1) in iron-sufficient conditions. In bioreactors inoculated with iron-starved pre-cultures, initial specific growth rates in batch culture were dependent on the iron concentration. The critical dilution rate (Dcrit, at which steady state cultures cannot be sustained) in continuous cultures was also dependent on the iron concentration and was lower than mu(max) in batch culture. Sucrose was the best carbon source for RA production [287+/-11 micromol (g biomass)(-1)] and production could be further increased by supplementing the medium with the precursors acetate [460+/-13 micromol (g biomass)(-1)], ornithine [376+/-6 micromol (g biomass)(-1)], or both [539+/-15 micromol (g biomass)(-1)]. Citric acid was an effective suppresser of RA production. RA was produced in a growth rate dependent manner and was optimally produced at pH 6.5.
AB - Rhodotorula mucilaginosa produces the siderophore rhodotorulic acid (RA) when grown in iron-limited conditions. R. mucilaginosa grew at rates between 0.10 and 0.19 h(-1) in iron-restricted conditions, depending on the carbon source, and at 0.23 h(-1) in iron-sufficient conditions. In bioreactors inoculated with iron-starved pre-cultures, initial specific growth rates in batch culture were dependent on the iron concentration. The critical dilution rate (Dcrit, at which steady state cultures cannot be sustained) in continuous cultures was also dependent on the iron concentration and was lower than mu(max) in batch culture. Sucrose was the best carbon source for RA production [287+/-11 micromol (g biomass)(-1)] and production could be further increased by supplementing the medium with the precursors acetate [460+/-13 micromol (g biomass)(-1)], ornithine [376+/-6 micromol (g biomass)(-1)], or both [539+/-15 micromol (g biomass)(-1)]. Citric acid was an effective suppresser of RA production. RA was produced in a growth rate dependent manner and was optimally produced at pH 6.5.
UR - http://www.scopus.com/inward/record.url?scp=0141504331&partnerID=8YFLogxK
U2 - 10.1017/S0953756203008220
DO - 10.1017/S0953756203008220
M3 - Article
C2 - 14531617
VL - 107
SP - 949
EP - 956
JO - Mycological Research
JF - Mycological Research
ER -