Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity

C.A. Barnes; A. Elsaesser; G. McKerr; C.V. Howard; J. Arkusz; A. Smok; J. Palus; E. Dziubaltowska; M. Stȩpnik; K. Rydzyński; A. Leśniak; A. Salvati; I. Lynch; K.A. Dawson; J.P. Hanrahan; W.H. De Jong

doi:10.1021/nl801661w

Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity

C.A. Barnes, A. Elsaesser, G. McKerr, C.V. Howard, J. Arkusz, A. Smok, J. Palus, E. Dziubaltowska, M. Stȩpnik, K. Rydzyński, A. Leśniak, A. Salvati, I. Lynch, K.A. Dawson, J.P. Hanrahan, W.H. De Jong

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239 Citations (Scopus)

Abstract

Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 μg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.

Original language	English
Pages (from-to)	3069-3074
Number of pages	6
Journal	Nano Letters
Volume	8
Issue number	9
DOIs	https://doi.org/10.1021/nl801661w
Publication status	Published - 1 Sept 2008

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Barnes, C. A., Elsaesser, A., McKerr, G., Howard, C. V., Arkusz, J., Smok, A., Palus, J., Dziubaltowska, E., Stȩpnik, M., Rydzyński, K., Leśniak, A., Salvati, A., Lynch, I., Dawson, K. A., Hanrahan, J. P., & De Jong, W. H. (2008). Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity. Nano Letters, 8(9), 3069-3074. https://doi.org/10.1021/nl801661w

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abstract = "Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 μg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.",

author = "C.A. Barnes and A. Elsaesser and G. McKerr and C.V. Howard and J. Arkusz and A. Smok and J. Palus and E. Dziubaltowska and M. Stȩpnik and K. Rydzy{\'n}ski and A. Le{\'s}niak and A. Salvati and I. Lynch and K.A. Dawson and J.P. Hanrahan and {De Jong}, W.H.",

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T1 - Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity

AU - Barnes, C.A.

AU - Elsaesser, A.

AU - McKerr, G.

AU - Howard, C.V.

AU - Arkusz, J.

AU - Smok, A.

AU - Palus, J.

AU - Dziubaltowska, E.

AU - Stȩpnik, M.

AU - Rydzyński, K.

AU - Leśniak, A.

AU - Salvati, A.

AU - Lynch, I.

AU - Dawson, K.A.

AU - Hanrahan, J.P.

AU - De Jong, W.H.

PY - 2008/9/1

Y1 - 2008/9/1

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AB - Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 μg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.

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Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity

Abstract

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