Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity

C.A. Barnes, A. Elsaesser, G. McKerr, C.V. Howard, J. Arkusz, A. Smok, J. Palus, E. Dziubaltowska, M. Stȩpnik, K. Rydzyński, A. Leśniak, A. Salvati, I. Lynch, K.A. Dawson, J.P. Hanrahan, W.H. De Jong

Research output: Contribution to journalArticlepeer-review

239 Citations (Scopus)

Abstract

Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 μg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.
Original languageEnglish
Pages (from-to)3069-3074
Number of pages6
JournalNano Letters
Volume8
Issue number9
DOIs
Publication statusPublished - 1 Sep 2008

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Copyright 2012 Elsevier B.V., All rights reserved.

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