Regulation of tumour necrosis factor α mRNA stability by the mitogen-activated protein kinase p38 signalling cascade

Matthew Brook; Gareth Sully; Andrew R. Clark; Jeremy Saklatvala

doi:10.1016/S0014-5793(00)02084-6

Regulation of tumour necrosis factor α mRNA stability by the mitogen-activated protein kinase p38 signalling cascade

Matthew Brook, Gareth Sully, Andrew R. Clark^*, Jeremy Saklatvala

^*Corresponding author for this work

Inflammation and Ageing

Research output: Contribution to journal › Article › peer-review

192 Citations (Scopus)

Abstract

The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine-rich element from the TNFα 3' untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	FEBS Letters
Volume	483
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)02084-6
Publication status	Published - 13 Oct 2000

Keywords

Lipopolysaccharide
Macrophage
Mitogen-activated protein kinase p38
mRNA stability
Translation
Tumor necrosis factor α

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)02084-6

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title = "Regulation of tumour necrosis factor α mRNA stability by the mitogen-activated protein kinase p38 signalling cascade",

abstract = "The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine-rich element from the TNFα 3' untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover. Copyright (C) 2000 Federation of European Biochemical Societies.",

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AU - Sully, Gareth

AU - Clark, Andrew R.

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N2 - The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine-rich element from the TNFα 3' untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine-rich element from the TNFα 3' untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Lipopolysaccharide

KW - Macrophage

KW - Mitogen-activated protein kinase p38

KW - mRNA stability

KW - Translation

KW - Tumor necrosis factor α

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Regulation of tumour necrosis factor α mRNA stability by the mitogen-activated protein kinase p38 signalling cascade

Abstract

Keywords

ASJC Scopus subject areas

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