Regulation of peptidoglycan synthesis by outer-membrane proteins

Athanasios Typas, Manuel Banzhaf, Bart van den Berg van Saparoea, Jolanda Verheul, Jacob Biboy, Robert J Nichols, Matylda Zietek, Katrin Beilharz, Kai Kannenberg, Moritz von Rechenberg, Eefjan Breukink, Tanneke den Blaauwen, Carol A Gross, Waldemar Vollmer

Research output: Contribution to journalArticlepeer-review

241 Citations (Scopus)


Growth of the mesh-like peptidoglycan (PG) sacculus located between the bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, and orchestrate division. Cytoskeletal elements direct placement and activity of PG synthases from inside the cell, but precise spatiotemporal control over this process is poorly understood. We demonstrate that PG synthases are also controlled from outside of the sacculus. Two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional PG synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new PG to the sacculus. LpoB shows partial septal localization, and our data suggest that the LpoB-PBP1B complex contributes to OM constriction during cell division. LpoA/LpoB and their PBP-docking regions are restricted to γ-proteobacteria, providing models for niche-specific regulation of sacculus growth.

Original languageEnglish
Pages (from-to)1097-109
Number of pages13
Issue number7
Publication statusPublished - 23 Dec 2010


  • Bacterial Outer Membrane Proteins
  • Cell Division
  • Cell Wall
  • Escherichia coli
  • Escherichia coli Proteins
  • Lipoproteins
  • Penicillin-Binding Proteins
  • Peptidoglycan
  • Peptidoglycan Glycosyltransferase
  • Protein Interaction Domains and Motifs
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't


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