Regulation of Cell Cycle and Productivity in NSO Cells by the Over-Expression of p21CIP1

Shikiko Watanabe, John Shuttleworth, Mohamed Al-Rubeai

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have constructed NS0 myeloma cell lines that inducibly express the p21CIP1 cyclin dependent kinase inhibitor, using the Lacswitch system. Ectopic p21(CIP1) protein expression was rapidly induced within 12 h of addition of IPTG, causing G1-phase arrest and almost complete inhibition of cell proliferation. The production of a chimeric IgG4 antibody, expressed constitutively from an independent promoter, was found to be significantly increased by more than 4-fold in p21CIP1-arrested cells. This study demonstrates for the first time the successful construction of anchorage-independent and proliferation-controlled NS0 cell lines with enhanced secreted chimeric antibody production independent of the inducible promoter activity used to achieve cytostasis.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiotechnology and Bioengineering
Volume77
Issue number1
Early online date7 Dec 2001
DOIs
Publication statusPublished - 5 Jan 2002

Keywords

  • proliferation
  • NS0 myeloma
  • p21(CIP1)
  • cell cycle
  • productivity

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