Regulated expression of leukocyte-specific transcript (LST) 1 in human intestinal inflammation

Jan Heidemann, Moritz Kebschull, Phil Robin Tepasse, Dominik Bettenworth

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


INTRODUCTION: Leukocyte-specific transcript 1 (LST1) encoded peptides are involved in immunomodulation and nanotube-mediated cell-cell communication. The aim of this study was to assess the expression of LST1 in colonic epithelium and endothelium during intestinal inflammation.

METHODS: LST1 expression was evaluated by RT-PCR, FACS, western blot analysis, and immunohistochemistry in intestinal epithelial Caco-2 cells, human intestinal microvascular endothelial cells and in human histological specimens from inflammatory bowel disease (IBD) patients and non-IBD colitis patients.

RESULTS: LST1 expression was significantly increased upon proinflammatory stimulation in intestinal epithelial and endothelial cells. Furthermore, LST1 tissue expression was significantly enhanced in macroscopically inflamed colonic mucosal biopsies as compared to non-affected mucosal areas.

CONCLUSIONS: This is the first report demonstrating regulated LST1 expression in human intestinal epithelial and microvascular endothelial cells and in inflamed colonic tissue from IBD patients. Proinflammatory expression of LST1 occurs in the setting of human IBD and is not restricted to immune cell populations. Future studies are needed to further elucidate the role of soluble and membrane-expressed LST1 in the regulation of mucosal intestinal immunity and inflammation as well as to reveal possible therapeutic implications.

Original languageEnglish
Pages (from-to)513-517
Number of pages5
JournalInflammation Research
Early online date30 Mar 2014
Publication statusPublished - Jul 2014


  • Adult
  • Anti-Inflammatory Agents, Non-Steroidal/adverse effects
  • Caco-2 Cells
  • Cells, Cultured
  • Colitis/etiology
  • Colon/metabolism
  • Diverticulitis/complications
  • Endothelial Cells/metabolism
  • Female
  • Gene Expression
  • Humans
  • Inflammatory Bowel Diseases/genetics
  • Intestinal Mucosa/metabolism
  • Male
  • Membrane Proteins/genetics
  • Middle Aged
  • Young Adult
  • LST1
  • Inflammation
  • TNF-α


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