Recirculating and germinal center B cells differentiate into cells responsive to polysaccharide-antigens

CG Vinuesa, DM-Y Sze, MC Cook, Kai-Michael Toellner, GGB Klaus, Jennifer Ball, Ian MacLennan

Research output: Contribution to journalArticle

51 Citations (Scopus)


Antibodies against bacterial capsular polysaccharides play a critical protective role. Responses to these antigens can occur without the help or control of T cells and are associated with marginal zone (MZ) B cells. Capsular antigens are diverse and some cross-react with self-carbohydrate epitopes. This diversity may explain the recruitment of non-autoreactive recirculating B cells and memory B cells to the MZ in addition to other B cells, some of which are weakly autoreactive cells, that are recruited to the MZ without entering the recirculating pool. To test whether memory B cells respond to polysaccharide-based antigens, mice with hapten-specific memory B cells were challenged with hapten-polysaccharide. Hapten-specific plasma cells producing high affinity antibody with Ig V-region mutations were induced. To test whether naive recirculating B cells can form MZ cells that respond to polysaccharide, recirculating B cells from lymph nodes were transferred into Rag-1-deficient mice. MZ cells differentiated from the donor cells without proliferation or T cell help and responded to polysaccharide-based antigen. The differentiation of B cells both from germinal centers and the recirculating pool to the MZ phenotype is likely to make an important contribution to the repertoire of B cells that respond to polysaccharide antigens.
Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalEuropean Journal of Immunology
Publication statusPublished - 1 Jan 2003


  • thymus-independent type 2 antigen
  • marginal zone B cell
  • recirculating B cell
  • memory B cell


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