Raster mode continuous-flow liquid microjunction mass spectrometry imaging of proteins in thin tissue sections

Rian Griffiths, Elizabeth Randall, Alan Race, Josephine Bunch, Helen Cooper

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)
158 Downloads (Pure)

Abstract

Mass spectrometry imaging by use of continuous-flow liquid microjunction sampling at discrete locations (array mode) has previously been demonstrated. In this Letter, we demonstrate continuous-flow liquid microjunction mass spectrometry imaging of proteins from thin tissue sections in raster mode, and discuss advantages (a tenfold reduction in analysis time) and challenges (suitable solvent systems, data interpretation) of the approach. Visualisation of data is non-trivial, requiring correlation of solvent-flow, mass spectral data acquisition rate, data quality and liquid microjunction sampling area. The latter is particularly important for determining optimum pixel size. The minimum achievable pixel size is related to the scan time of the instrument used. Here we show a minimum achievable pixel size of 50 µm (x-dimension) when using an Orbitrap Elite; however a pixel size of 600 µm is recommended in order to minimise the effects of oversampling on image accuracy.
Original languageEnglish
Pages (from-to)5683–5687
JournalAnalytical Chemistry
Volume89
Issue number11
Early online date11 May 2017
DOIs
Publication statusPublished - 6 Jun 2017

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